LOCUS       sequence-64071-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength GCaMP2-based genetically encoded calcium 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreanu
            L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, 
            Svoboda K, Looger LL.
  TITLE     Imaging neural activity in worms, flies and mice with improved GCaMP
            calcium indicators.
  JOURNAL   Nat Methods. 2009 Dec;6(12):875-81.
  PUBMED    19898485
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-12"; volume: "6(12)"; pages: "875-81"
COMMENT     Sequence Label: G-CaMP3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(317..373)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(401..415,416..424))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(428..460)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(464..481)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(547..750)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(576..596)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
      241 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccttga
      301 tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga cctgtcttat
      361 tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca
      421 gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga tgatgatgat
      481 gagaacccat catggtggcg agatctgagt ccggtagcgc tagcggatct gacggttcac
      541 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      601 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      661 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      721 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      781 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      841 tttaccgtca ttgacgtca
//
