LOCUS       sequence-64080-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS337-USP44-FLAG
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
       61 caccatcggg cgtgctagcc accatggact acaaggatga cgatgacaag catatgccca
      121 tgggatccga gctcgagatc tgcagctggt acccatcaac aagtttgtac aaaaaagttg
      181 gcgcgcccac catgctagca atggatacgt gcaaacatgt tgggcagctg cagcttgctc
      241 aagaccattc cagcctcaac cctcagaaat ggcactgtgt ggactgcaac acgaccgagt
      301 ccatttgggc ttgccttagc tgctcccatg ttgcctgtgg aagatatatt gaagagcatg
      361 cactcaagca ctttcaagaa agcagtcatc ctgttgcatt ggaggtgaat gagatgtacg
      421 ttttttgtta cctttgtgat gattatgttc tgaatgataa cgcaactgga gacctgaagt
      481 tactacgacg tacattaagt gccatcaaaa gtcaaaatta tcactgcaca actcgtagtg
      541 ggaggttttt acggtccatg ggtacaggtg atgattctta tttcttacat gacggtgccc
      601 aatctctgct tcaaagtgaa gatcaactgt atactgctct ttggcacagg agaaggatac
      661 taatgggtaa aatctttcga acatggtttg aacaatcacc cattggaaga aaaaagcaag
      721 aagaaccatt tcaggaaaaa atagtagtaa aaa
//
