LOCUS       sequence-64096-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Gubbels SP, Woessner DW, Mitchell JC, Ricci AJ, Brigande JV
  TITLE     Functional auditory hair cells produced in the mammalian cochlea by 
            in utero gene transfer.
  JOURNAL   Nature. 2008 Sep 25. 455(7212):537-41.
  PUBMED    18754012
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Sep 25. 455(7212):537-41."
COMMENT     Sequence Label: pEF-I-GFP GX
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          302..505
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(325..345)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg tttgataatt cctcacgaca
      301 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
      361 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
      421 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
      481 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
      541 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
      601 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc
      661 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
      721 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
      781 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
      841 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata
//
