LOCUS       sequence-64113-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Mann RK, Grunstein M
  TITLE     Histone H3 N-terminal mutations allow hyperactivation of the yeast 
            GAL1 gene in vivo.
  JOURNAL   EMBO J. 1992 Sep;11(9):3297-306.
  PUBMED    1505519
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1992-09"; volume: "11(9)"; pages: "3297-306"
COMMENT     Sequence Label: pRM200U
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(753..769)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(753..769)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(801..820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtccggta gaggtaaagg tggtaaaggt ctaggtaaag gtggtgccaa gcgtcacaga
       61 aagattttaa gagataacat tcaaggtatc actaagccag ctatcagaag attagctaga
      121 agaggtggtg tcaagcgtat ttctggtttg atctacgaag aagtcagagc cgtcttgaaa
      181 tccttcttgg aatccgtcat cagggactct gttacttaca ctgaacacgc caagagaaag
      241 actgttactt ctttggatgt tgtttatgct ttgaagagac aaggtagaac cttatatggt
      301 ttcggtggtt aaacaatcgg tggttaaaca atcggtgttt gaaattattt tcatgccttt
      361 caaaaaataa aataacgatc attctattgg gaatataata gaaccttatg tatgcatttt
      421 tcggtttttt attatttcat gtactatata tattaaaaat aagtctcaat aaaagaaact
      481 agtttccgaa ctgtcttaga tagtcttccc ttatgtgctc ctccatttca ttgccgttga
      541 gtgcttcgac gtggcgttcg tgtcccctct gttggttaag cagatttacc caaatattta
      601 cgcagaaacc catggaatcc tcaatttcac gctttttctt gggacatctg cacctgcagc
      661 cacacccaaa ttcccttccc tcgtcgtagg ccgcgttttt acgtttgaat tttgagtcac
      721 tggctaagta ggagaattcc tgcagcccgg gggatccact agttctagag cggccgccac
      781 cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgt
//
