LOCUS       sequence-64121-e        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Tian L, Yang Y, Wysocki LM, Arnold AC, Hu A, Ravichandran B, 
            Sternson SM, Looger LL, Lavis LD
  TITLE     Selective esterase-ester pair for targeting small molecules with 
            cellular specificity.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4756-61. doi: 
            10.1073/pnas.1111943109. Epub 2012 Mar 12.
  PUBMED    22411832
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1111943109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-27- 27"; volume: "109"; issue: "13"; pages: "4756-61"
COMMENT     Sequence Label: pCAG-PLE-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    join(3506..4080,4081..4083,4084..4092)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3673..3690)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3900..3919
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4099..4119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4120..4827
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4262..4280)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4530..4549
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4724..4747
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     intron          4885..5017
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5000..5019
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5026..5614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5079..5099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5497..5508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5641..5658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5647..5758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5985..6004)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6050..6105
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6104..6123)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6464..6480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6477..6499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6488..6504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6512..6518,6519..6536,6537..6542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6557..6578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6637..6832
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6683..6818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6838..6972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6875..6894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6929..6948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7040..7057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7211..7799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7291..7310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7970..8761,8762..8830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8593..8612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8831..8935)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcggtacc gcgggcccgg
     1741 cgcgccagct ggatatctcc ggacccgggc tagcgcaatt gcggccgcca ccatgtggct
     1801 gctgcctctg gtgctgacca gcctggccag cagcgccacc tgggccggcc agcccgccag
     1861 ccctcccgtg gtggacaccg cccagggcag ggtgctgggc aagtacgtga gcctggaggg
     1921 cctggcccag cccgtggccg tgttcctggg cgtgcccttc gccaagcctc ccttgggcag
     1981 cctgaggttc gctcctcctc agcctgctga gccctggagc ttcgtgaaga acaccaccag
     2041 ctaccctccc atgtgctgcc aggatcccgt ggtggagcag atgaccagcg acctgttcac
     2101 caacggcaag gagaggctga ccctggagtt cagcgaggac tgcctgtacc tgaacatcta
     2161 cacacccgcc gacctgacca agagaggcag gctgcccgtg atggtgtgga tccacggcgg
     2221 cggcctggtg ctgggcggcg ctcccatgta cgacggcgtg gtgctggccg cccacgagaa
     2281 cgtggtggtg gtggccatcc agtacaggct gggcatctgg ggcttcttca gcaccggcga
     2341 cgagcacagc aggggcaact ggggccacct ggaccaggtg gccgccctgc actgggtgca
     2401 ggagaacatc gccaacttcg gcggcgatcc cggcagcgtg accatcttcg gcgagagcgc
     2461 cggcggcgag agcgtgagcg tgctggtgct gagccctctg gccaagaacc tgttccacag
     2521 ggccatcagc gagagcggcg tggccctgac cgtggccctg gtgaggaagg acatgaaggc
     2581 cgccgccaag cagatcgccg tgctggccgg ctgcaagacc accaccagcg ccgtgttcgt
     2641 gcactgcctg aggcagaaga gcgaggacga gctgctggac ctgaccctga agatgaagtt
     2701 cctgaccctg gacttccacg gcgaccagag ggagagccat cccttcctgc ccaccgtggt
     2761 ggacggcgtg ctgctgccca agatgcccga ggagatcctg gccgagaagg acttcaacac
     2821 cgtgccctac atcgtgggca tcaacaagca ggagttcggc tggctgctgc ccactatgat
     2881 gggcttccct ctgagcgagg gcaagttgga ccagaagacc gccaccagcc tgctgtggaa
     2941 gagctatccc atcgccaaca ttcccgagga gctgacaccc gtggccaccg acaagtacct
     3001 gggcggcacc gacgatcccg tgaagaagaa ggacctgttc ctggacctga tgggcgacgt
     3061 ggtgttcggc gtgcccagcg tgaccgtggc caggcagcac agggacaccg gcgctcccac
     3121 ctacatgtac gagttccagt acaggcccag cttcagcagc gacaagaagc ccaagtccgt
     3181 gatcggcgac cacggcgacg agatcttcag cgtgttcggc ttccctctgc tgaagggcga
     3241 cgctcccgag gaggaggtga gcctgagcaa gaccgtgatg aagttctggg ccaacttcgc
     3301 caggagcggc aatcccaacg gcgagggcct gcctcactgg cccatgtacg accaggagga
     3361 gggctacctg cagatcggcg tgaacaccca ggccgccaag aggctgaagg gcgaggaggt
     3421 ggccttctgg aacgacctgc tgagcaagga ggccgccaag aagcctccta agatcaagca
     3481 cgccgagctg taaaccggaa ttccgcccct ctccctcccc cccccctaac gttactggcc
     3541 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3601 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     3661 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     3721 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     3781 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     3841 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     3901 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggca ccccattgta
     3961 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4021 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     4081 atggccacaa ccatggtgcc caagaagaag aggaaagtcg tgagcaaggg cgaggaggac
     4141 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     4201 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     4261 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtcccct
     4321 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     4381 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     4441 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     4501 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4561 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4621 aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacaaggcc
     4681 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagctgga catcacctcc
     4741 cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc
     4801 ggcggcatgg acgagctgta caagtaaatg cagaacggcc ccggtgctcg aggtaccgat
     4861 gaatagctaa ggtcgaggcc gcaggtaagt atcaaggtta caagacaggt ttaaggagac
     4921 caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
     4981 ggtcttactg acatccactt tgcctttctc tccacaggtg tcgacaatca acctctggat
     5041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5401 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5581 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacgatcag
     5641 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5701 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5761 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5821 aggattggga agacaatagc aggccagctt ttgttccctt tagggttaat tgcgcgcttg
     5881 gcgctaatca tggtcatagc tgtttccact cctcaggtgc aggctgccta tcagaaggtg
     5941 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     6001 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     6061 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     6121 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     6181 gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt atatgaaaca
     6241 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     6301 ttatattttg tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     6361 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     6421 atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     6481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6541 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6601 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     6661 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6721 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6781 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     6841 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6901 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6961 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7021 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7081 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7141 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7201 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7381 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     7441 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7501 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7561 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7621 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7681 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7741 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7801 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7861 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7921 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7981 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8041 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8101 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8161 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8221 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8281 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8341 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8401 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8461 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8521 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8581 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8641 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8701 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8761 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8821 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8881 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8941 ttccccgaaa agtgccacct g
//
