LOCUS       sequence-64124-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Hecht A, Laroche T, Strahl-Bolsinger S, Gasser SM, Grunstein M
  TITLE     Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a 
            molecular model for the formation of heterochromatin in yeast.
  JOURNAL   Cell. 1995 Feb 24;80(4):583-92.
  PUBMED    7867066
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-02-24"; volume: "80(4)"; pages: "583-92"
COMMENT     Sequence Label: p107.1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(146..799)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(166..188)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(811..833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    822..838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(846..852,853..868,869..874))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 agtcagtcac gatgaattca gctggcttag tgataccttg aatgttatct cttagaatct
       61 ttctgtgacg cttggcacca ccttttccta gacctttacc acctttacct ctaccggaca
      121 tggatccacg cggaaccaga tccgattttg gaggatggtc gccaccacca aacgtggctt
      181 gccagccctg caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg
      241 ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg
      301 ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat
      361 gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag
      421 gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg
      481 aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt
      541 ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact
      601 gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc aaacccaatt
      661 caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc tcttcatatt
      721 tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta attttccaat
      781 aacctagtat aggggacatg aatactgttt cctgtgtgaa attgttatcc gctcacaatt
      841 ccacacatta tacgagccga tgattaattg tcaacagctc at
//
