LOCUS       sequence-64126-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hecht A, Laroche T, Strahl-Bolsinger S, Gasser SM, Grunstein M
  TITLE     Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a 
            molecular model for the formation of heterochromatin in yeast.
  JOURNAL   Cell. 1995 Feb 24;80(4):583-92.
  PUBMED    7867066
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-02-24"; volume: "80(4)"; pages: "583-92"
COMMENT     Sequence Label: p296.2
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(141..158)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(165..818)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(185..207)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaggcagatc gtcagtcagt cacgatgaat tctagcaatc tgtgcactct accgactggg
       61 aatgtcaaac cagccttagc agatctagat tgagaagctt tagcagctga accagcttta
      121 ccacctttac caccggacat ggatccacgc ggaaccagat ccgattttgg aggatggtcg
      181 ccaccaccaa acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc
      241 aagtacttat caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg
      301 aacgcatcca ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg
      361 aagtcaggat gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg
      421 aacattttca gcatttcagg tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct
      481 ttactatatg caattctcga aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt
      541 gaaatctctg cacgctcttt tggacaacca cccaacatgt tgtgcttgtc agctatataa
      601 cgtatgatgg ccatagactg tgttaattta acatcaccat caatataata aggaagattg
      661 ggaaactcca aacccaattc aaactttttg tttcgccatt tatcaccttc atcgcgctca
      721 tacaaatgct cttcatattt ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca
      781 aggcccttaa ttttccaata acctagtata ggggacatga atactgtttc ctgtgt
//
