LOCUS       sequence-64127-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Hecht A, Laroche T, Strahl-Bolsinger S, Gasser SM, Grunstein M
  TITLE     Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a 
            molecular model for the formation of heterochromatin in yeast.
  JOURNAL   Cell. 1995 Feb 24;80(4):583-92.
  PUBMED    7867066
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-02-24"; volume: "80(4)"; pages: "583-92"
COMMENT     Sequence Label: p297.1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..164)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(171..818)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="PILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFE
                     LGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGV
                     SRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM
                     DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(191..213)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 acgcgcgagg cagatcgtca gtcagtcacg atgaattcaa ccttagatct cttcttacca
       61 tcgacggagg ttgatgtttt cttggcagct ggcttctttt cagctggagc tttggaagct
      121 ggtttctttt cggcggcaga ggacatggat ccacgcggaa ccagatccga ttttggagga
      181 tggtcgccac caccaaacgt ggcttgccag ccctgcaaag gccatgctat atacttgctg
      241 gatttcaagt acttatcaat ttgtgggata gcttcaatac gttttttaaa acaaactaat
      301 tttgggaacg catccaggca cattgggtcc atgtataaaa caacatcaag agcgtcatac
      361 aacatgaagt caggatgggt tacatgatca ccatttaaat atgttttatg acataaacga
      421 tcttcgaaca ttttcagcat ttcaggtagc ttgctaagaa aatcaacttt gagagtttca
      481 aagtctttac tatatgcaat tctcgaaaca ccgtatctaa tatccaaaac cgctccttca
      541 agcattgaaa tctctgcacg ctcttttgga caaccaccca acatgttgtg cttgtcagct
      601 atataacgta tgatggccat agactgtgtt aatttaacat caccatcaat ataataagga
      661 agattgggaa actccaaacc caattcaaac tttttgtttc gccatttatc accttcatcg
      721 cgctcataca aatgctcttc atatttttct tcaagatatt ccaaaagaag tcgagtgggt
      781 tgcacaaggc ccttaatttt ccaataacct agtataggg
//
