LOCUS       sequence-64153-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     BC-GPR56
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BC-Sig-GPRC
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..91)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(98..115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(98..114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(107..129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataaccg
       61 gaattaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      121 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      181 ccagaatttc gaccactgtg ctggcggccg ctcattacta gatgcggctg gacgaggtgc
      241 tgcccgagct gatggggagc ctggcgctgt ctgagttgct cttcagaggg gaggggccac
      301 cccgggcctg cagccgcatg gaccagtacc agatgaagat gaggaagcct tggaaggagg
      361 tgatgatgct gaaaaggtag aggacgacaa gctggaaggt gccagaagca aaggagaaga
      421 agatcaaggc ccagggcagg ccaaggacca ggctgaggcc cagcagtgtc agcacatgtg
      481 accacttttg ggtgtggggg cgcagccgca ggatctgcac caccatggtg gctagcatgg
      541 ccatgttgaa cagaaacacc aggctgaaga ggcccaggtt ggtgatgtag ctgaccaggg
      601 agtcccggat ccagcacatg gaagggtaga tgacgccctc tggagtccta tgcacagcca
      661 agatgatggg gccatagttg tccacatcca ccagggccac cagcgtcacc agaaagatgg
      721 ggaagcccca gcccatggcg ctcagcttga gtaggtagcc agggacatag gtgccaaaga
      781 cctccaccac gagtcggtag aggttgtacc cctcgaggcc catccaggaa aggcaggtga
      841 gcagggagaa gtgcaggaag atggcactgg ctcggcagcc agcctcagag cctgtcaggg
      901 ccaccggctc gctgagcagg aagctcgtgt c
//
