LOCUS       sequence-64168-e        1187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Chaudhuri AA, So AY, Mehta A, Minisandram A, Sinha N, Jonsson VD, 
            Rao DS, O'Connell RM, Baltimore D
  TITLE     Oncomir miR-125b regulates hematopoiesis by targeting the gene 
            Lin28A.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4233-8. doi: 
            10.1073/pnas.1200677109. Epub 2012 Feb 24.
  PUBMED    22366319
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1200677109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-13- 13"; volume: "109"; issue: "11"; pages: "4233-8"
COMMENT     Sequence Label: MG-miR125b-sponge-bulge
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..61
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(109..111,112..114,115..828)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMXELYK"
     primer_bind     complement(154..175)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(415..434)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     762..783
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     gap             1138..1148
                     /estimated_length=11
ORIGIN
        1 cnnntctccc ttganctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
       61 ctccttctct aggcgccgga attagatccc cgggtaccgg tcgccaccat ggtgagcaag
      121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      301 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      781 gtgaccgccg ccgggatcac tctcggcatg gncgagctgt acaagtaaag cgggccgctc
      841 acaagttacc actcagggnc gattcacaag ttaccactca ggaanccngn ncacaagtta
      901 ccactcaggg atcactcaca agttaccact canggactcg aggttaacga atttcgacct
      961 gcagcnagct tatcgatnaa aataaaagan tttnnnttag tntncagaaa aaaggggggg
     1021 aatgaaagna cccnncctgn aggntnnnng ntancttaag nannncattt tgcangcntg
     1081 aaanncnnan tgnnnannna nnnnnnnatc agnngancnn annnanngcn nannnggnnn
     1141 nnnnnnnntn nnnannnntn cnnncncngn tnaggnnana annnnaa
//
