LOCUS       sequence-64180-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Lee HY, Haurwitz RE, Apffel A, Zhou K, Smart B, Wenger CD, Laderman 
            S, Bruhn L, Doudna JA
  TITLE     RNA-protein analysis using a conditional CRISPR nuclease.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5416-21. doi: 
            10.1073/pnas.1302807110. Epub 2013 Mar 14.
  PUBMED    23493562
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1302807110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-2- 2"; volume: "110"; issue: "14"; pages: "5416-21"
COMMENT     Sequence Label: Csy4H29AS50C
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             77..640
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLAQALVAQGGDRIGVSFP
                     DLDECRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     protein_bind    complement(660..684)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             698..715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(768..786)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tcacaagttt gtacaaaaag caggctccgc ggccgccgta ctcgaggaaa acctgtattt
       61 tcagggctcc ttcaccatgg accactacct cgacattcgc ttgcgaccgg acccggaatt
      121 tcccccggcg caactcatga gcgtgctctt cggcaagctc gcccaggccc tggtggcaca
      181 gggcggggac aggatcggcg tgagcttccc cgacctcgac gaatgccgct cccggctggg
      241 cgagcgcctg cgcattcatg cctcggcgga cgaccttcgt gccctgctcg cccggccctg
      301 gctggaaggg ttgcgggacc atctgcaatt cggagaaccg gcagtcgtgc ctcaccccac
      361 accgtaccgt caggtcagtc gggttcaggc gaaaagcaat ccggaacgcc tgcggcggcg
      421 gctcatgcgc cggcacgatc tgagtgagga ggaggctcgg aaacgcattc ccgatacggt
      481 cgcgagagcc ttggacctgc ccttcgtcac gctacgcagc cagagcaccg gacagcactt
      541 ccgtctcttc atccgccacg ggccgttgca ggtgacggca gaggaaggag gattcacctg
      601 ttacgggttg agcaaaggag gtttcgttcc ctggttctga taaaagggtg ggcgcgccga
      661 cccagctttc ttgtacaaag tggtgatgta cctcgagcac caccaccacc accactgaga
      721 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      781 actagcataa ccccttgggc tctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      841 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg cccta
//
