LOCUS       sequence-64183-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Nowotschin S, Xenopoulos P, Schrode N, Hadjantonakis AK
  TITLE     A bright single-cell resolution live imaging reporter of Notch 
            signaling in the mouse.
  JOURNAL   BMC Dev Biol. 2013 Apr 25;13:15. doi: 10.1186/1471-213X-13-15.
  PUBMED    23617465
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2013-04-25"; volume: "13:15. doi"; pages: " 
            10.1186/1471-213X-13-15"
COMMENT     Sequence Label: CBF:H2B-Venus
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(144..395)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(186..207)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     535..554
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      121 gctcgagagg ccttgaattc ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      181 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      241 gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      301 gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      361 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      421 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      481 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      541 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      601 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg
      661 tagcccaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc
      721 agcttcaggg tcagcttgcc g
//
