LOCUS       sequence-64201-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     Cohen Lab VIP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJMK019
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..783
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYANWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             811..828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actctagaga tggaccccat cgctctgcag gctggttacg acctgctggg tgacggcaga
       61 cctgaaactc tgtggctggg catcggcact ctgctgatgc tgattggaac cttctacttt
      121 ctggtccgcg gatggggagt caccgataag gatgcccggg aatattacgc tgtgactatc
      181 ctggtgcccg gaatcgcatc cgccgcatat ctgtctatgt tctttggtat cgggcttact
      241 gaggtgaccg tcgggggcga aatgttggat atctattatg ccaggtacgc caactggctg
      301 tttaccaccc cacttctgct gctggatctg gcccttctcg ctaaggtgga tcgggtgacc
      361 atcggcaccc tggtgggtgt ggacgccctg atgatcgtca ctggcctcat cggagccttg
      421 agccacacgg ccatagccag atacagttgg tggttgttct ctacaatttg catgatagtg
      481 gtgctctatt ttctggctac atccctgcga tctgctgcaa aggagcgggg ccccgaggtg
      541 gcatctacct ttaacaccct gacagctctg gtcttggtgc tgtggaccgc ttaccctatc
      601 ctgtggatca taggcactga gggcgctggc gtggtgggcc tgggcatcga aactctgctg
      661 tttatggtgt tggacgtgac tgccaaggtc ggctttggct ttatcctgtt gagatcccgg
      721 gctattctgg gcgacaccga ggcaccagaa cccagtgccg gtgccgatgt cagtgccgcc
      781 gaccgaaccg gtagtggcgc atcaggttct catcatcatc atcatcatgg t
//
