LOCUS       sequence-64206-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kanther M, Sun X, Muhlbauer M, Mackey LC, Flynn EJ 3rd, Bagnat M, 
            Jobin C, Rawls JF
  TITLE     Microbial colonization induces dynamic temporal and spatial patterns
            of NF-kappaB activation in the zebrafish digestive tract.
  JOURNAL   Gastroenterology. 2011 Jul;141(1):197-207. doi: 
            10.1053/j.gastro.2011.03.042. Epub 2011 Mar 24.
  PUBMED    21439961
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2011.03.042"; journalName: "Gastroenterology"; 
            date: "2011-07"; volume: "141"; issue: "1"; pages: "197-207"
COMMENT     Sequence Label: pNFkB:eGFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..97
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    263..287
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(425..449)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctcgccctt gctcaccatg gtggcgaccg gtggatcctc gaagtttggg gaaagcccgg
       61 caaggggtcc aggggtagac actggtggga gctgcagagc agagctgggt gggagcgcgg
      121 tcactgctcg ttcgcggaac cgccggctct atccagtctt ctcagttgct cgctgcagtc
      181 gcggttggag tagtaggcgc ctcagctggc gcctttatag aagcgctgtg aatggatgga
      241 cttcctacgt cactggatat caaccacttt gtacaagaaa gctgggtcga gctcaagctt
      301 cgaattctgc aggtcgacgg aaagtcccca gcggaaagtc cctcgacgga aagtccccag
      361 cggaaagtcc ctcgacggaa agtccccagc ggaaagtccc tcgaccgtgc atgcattcac
      421 aggaagcctg cttttttgta caaacttgtt gatatctcga gggcccatct ggcctgtgtt
      481 tcagacacca gggagtctct gctcacgttt cctgctattt gcagcctctc tatcaagact
      541 aatacacctc ttcccgcatc ggctgcctgt gagaggcttt tcagcactgc aggattgctt
      601 ttcagcccca aaagagctag gcttgacact aacaattttg agaatcagct tctactgaag
      661 ttaaatctga ggttttacaa ctttgagtag cgtgtactgg cattagattg tctgtcttat
      721 agtttgataa ttaaatacaa acagttctaa agcaggataa aaccttgtat gcatttcatt
      781 taatgttttt tgagattaaa agcttaaaca agaatctcta gttttctttc ttgcttttac
      841 ttttacttcc ttaatactca agtacaattt taatggagta cttttttact tttactcaag
      901 taagattcta gccagatact tttactttta attgagtaaa attttcccta a
//
