LOCUS       sequence-64210-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pWzl_neo_DEST_flag_hcRED
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             81..764
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(766..790)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggatccaga attcgccacc atggactaca aagacgatga cgacaagcaa ttgacaagtt
       61 tgtacaaaaa agttggcacc atggtgagcg gcctgctgaa ggagagtatg cgcatcaaga
      121 tgtacatgga gggcaccgtg aacggccact acttcaagtg cgagggcgag ggcgacggca
      181 accccttcgc cggcacccag agcatgagaa tccacgtgac cgagggcgcc cccctgccct
      241 tcgccttcga catcctggcc ccctgctgcg agtacggcag caggaccttc gtgcaccaca
      301 ccgccgagat ccccgacttc ttcaagcaga gcttccccga gggcttcacc tgggagagaa
      361 ccaccaccta cgaggacggc ggcatcctga ccgcccacca ggacaccagc ctggagggca
      421 actgcctgat ctacaaggtg aaggtgcacg gcaccaactt ccccgccgac ggccccgtga
      481 tgaagaacaa gagcggcggc tgggagccca gcaccgaggt ggtgtacccc gagaacggcg
      541 tgctgtgcgg ccggaacgtg atggccctga aggtgggcga ccggcacctg atctgccacc
      601 actacaccag ctaccggagc aagaaggccg tgcgcgccct gaccatgccc ggcttccact
      661 tcaccgacat ccggctccag atgctgcgga agaagaagga cgagtacttc gagctgtacg
      721 aggccagcgt ggcccggtac agcgacctgc ccgagaaggc caacttgcca actttcttgt
      781 acaaagtggt ttgatctagt cgaccaattc cggttatttt ccaccatatt gccgtctttt
      841 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgag
//
