LOCUS       sequence-64212-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1 (220aa)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..123)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(810..834)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(857..907)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(886..906)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg
       61 gttagcgcta gctcattact acgtagaatc gagaccgagg agagggttag ggataggctt
      121 accaaccact ttgtacaaga aagttgggta tgatttttca tgtgaagatt taggtgtttc
      181 tccatcattt tcctcttcct catcctcttc acaatcccca tctgtttgct gttccagttc
      241 ctcttttgtt atcatttcaa aatcatttcc atccagaaaa acctgatcat catgtggctc
      301 aagtttgaat tttttcttag tttccttctt tttaggagtt cctggagttt cctccttttt
      361 ggattcttcc ttttcaccat cttttttttt ctctttttct ttttttgtct tctcatcctt
      421 tatattttct ttcttgctct ttttatcttc ttcttttcca ctttcagctt ttcctttatc
      481 tttttctttc tttatgtctt tatcatattt cattttcatt acttttaggg ctcggtgaaa
      541 aaactgcttc tttaaaagcc tgttgcagta gtcaaccaca gactccctgg ttgtaaataa
      601 agcttcctct cctttcttgg cctttgccca ctttgaatcc aaaagacagt ccactgcttt
      661 tgaagcaata aaataatcaa cccggtgacc catcatattg gtggactttg ttggacagtt
      721 gaatcgaaga tacttggcca cagccttctc ttctttagat ggttcaccaa cttcctggat
      781 ccgcttcttg tgtctcctgc gttccgccat gccaactttt ttgtacaaac ttgttgatcc
      841 cgacagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      901 tttgcgt
//
