LOCUS       sequence-64220-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_BFP
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(82..123)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(151..861,862..864,865..867))
                     /codon_start=1
                     /product="blue variant of GFP"
                     /label=BFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(193..214)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     542..561
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     801..822
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg
       61 gttagcgcta gctcattact acgtagaatc gagaccgagg agagggttag ggataggctt
      121 accaaccact ttgtacaaga aagttggcaa cttgtacagc tcgtccatgc cgagagtgat
      181 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      241 gctcagggcg gactgggtgc tcaggtagtg gctgtcgggc agcagcacgg ggccgtcgcc
      301 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      361 gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      421 gtggctgttg aagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      481 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      541 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      601 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
      661 gacgccgtgg ctcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      721 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      781 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      841 gaacagctcc tcgcccttgc tcaccatggt gc
//
