LOCUS       sequence-64226-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pWzl_neo_DEST_flag_PIK3CA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccggatcca gaattcgcca ccatggacta caaagacgat gacgacaagc aattgacaag
       61 tttgtacaaa aaagttggca ccatgcctcc acgaccatca tcaggtgaac tgtggggcat
      121 ccacttgatg cccccaagaa tcctagtaga atgtttacta ccaaatggaa tgatagtgac
      181 tttagaatgc ctccgtgagg ctacattaat aaccataaag catgaactat ttaaagaagc
      241 aagaaaatac cccctccatc aacttcttca agatgaatct tcttacattt tcgtaagtgt
      301 tactcaagaa gcagaaaggg aagaattttt tgatgaaaca agacgacttt gtgaccttcg
      361 gctttttcaa ccctttttaa aagtaattga accagtaggc aaccgtgaag aaaagatcct
      421 caatcgagaa attggttttg ctatcggcat gccagtgtgt gaatttgata tggttaaaga
      481 tccagaagta caggacttcc gaagaaatat tctgaacgtt tgtaaagaag ctgtggatct
      541 tagggacctc aattcacctc atagtagagc aatgtatgtc tatcctccaa atgtagaatc
      601 ttcaccagaa ttgccaaagc acatatataa taaattagat aaagggcaaa taatagtggt
      661 gatctgggta atagtttctc caaataatga caagcagaag tatactctga aaatcaacca
      721 tgactgtgta ccagaacaag taattgctga agcaatcagg aaaaaaactc gaagtatgtt
      781 gctatcctct gaacaactaa aactctgtgt tttagaatat cagggcaagt atattttaaa
      841 agtgtgtgga tgtgatgaat acttcctaga aaa
//
