LOCUS       sequence-64227-e        7765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Loewer S, Cabili MN, Guttman M, Loh YH, Thomas K, Park IH, Garber M,
            Curran M, Onder T, Agarwal S, Manos PD, Datta S, Lander ES, 
            Schlaeger TM, Daley GQ, Rinn JL
  TITLE     Large intergenic non-coding RNA-RoR modulates reprogramming of human
            induced pluripotent stem cells.
  JOURNAL   Nat Genet. 2010 Dec;42(12):1113-7. Epub 2010 Nov 7.
  PUBMED    21057500
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2010-12"; volume: "42(12)"; pages: "1113-7. Epub 
            2010 Nov 7"
COMMENT     Sequence Label: pBabe-lincRNA-RoR
FEATURES             Location/Qualifiers
     source          1..7765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4004..4024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4009..4338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4189..4324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4348..4947
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4348..4367)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4844..4864
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5046..5639
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5840..5857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6011..6599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6091..6110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6777..7568,7569..7637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7400..7419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7638..7742)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
     1381 attcggtgaa ataaacagcc atgttgctca cacaaagcct gtttggtggt ctcttcacac
     1441 ggacgctcat gaaatttggt gatgtgactc ggataggggg acctcccttg ggagatcagt
     1501 accctgtcct cctgctcttt gctccgtgag aaagatccac ctacaacctc aggtccttag
     1561 accaaccagc ccaagaaaca tctcaccaat ttcaaatcca gaccccactg gaaatcggac
     1621 tgtccaactc acctgacagc cactcccaca gccgctggaa ctctggccca aggctctctg
     1681 actccttccc agatcttctt ggcttagcgg ctgaagactg acgctgcccg atcgcctcgg
     1741 aagcccccta gaccatcacg gacgccgagc ttcgggtaac tctcacagtg gaagaaacac
     1801 aactacagat ttctacctgg tgcatggcca tcgctcatga agactacaac ttccagcttc
     1861 ctttgaagaa aaagaggact tgatggcatt gtcgctaagt aagaaataat gtgtgtgact
     1921 tcagggttgc ccccttaaag ggaggggaca ttttccatcc tgctgttcag agtatggatg
     1981 tgatgagaga catcttggat gatgcagagg aggtgaacac cccaggacaa tgaaaccaca
     2041 ggagagaaag agcctgcgct ggcccatcta gcacagccac tggacacagg gaccacctgc
     2101 ctctgcactc ttatggaagg aggaaatcta actttcccag tttaaggcac tgttactttg
     2161 ggctcctgtt acataactgt ggcagaatga aggttcaaca tggaaactgg caatgttgaa
     2221 gaaacataaa gttcattgct tgaatatctg aagttgtgga ctcaatctca tacctgctcc
     2281 acttatgagt tatagttctt ccaggtctca ggaatgggat cagcaggtct cagggttgta
     2341 ctctcctgga tctctcacca gccacctcaa accagctgcc atagcctgtc cacttccact
     2401 ccaatcttct cttccttcat cacctccctt gcacaccctg ataacctcga aagagaactc
     2461 ttcccaaggc tctgttccaa acacatcgcc actctgctta gaaccttcaa tgactcctca
     2521 tggcctagga ggtttctctc ccatctggat ccagctgacg ttcccagcac cttctcctga
     2581 ctcctgtctt ttcttgaacc agttctgccc aacaaggagg aaagggctga cagagtgaaa
     2641 gtcccagggc atgtgggaat gtgactcttt tcactttaaa ttctatgact ggaaagtttt
     2701 gggcagagtt ggacatgtgc acttagcttc cagaagacag aatcctttta aaagagtcag
     2761 agaaaacact ggcttcctgc catgacatga gatacagaca ggagagttgg gaagcttttt
     2821 aaagatggca ctatgactac aatcacagaa actctccatg aggaagtaaa agaaagcacc
     2881 tgcaacactc cagctatgca gaccactctg taatgggctc agatctggac aggtgtgtgg
     2941 aaaggtgggt caacaggtca ggcgtcacag acttggaaca ttcatggtgg aaaagaaaaa
     3001 gccccaaaga agagacttca ggataaatga gaaaatactc aagacagcaa aagtctcttt
     3061 tagaaatgtt ggagaaagaa cacttaatgt caggagttac tgttgattga tggccttact
     3121 gtgtagcagg tgagaaaccc attgttcagt tccctaaagt caccctattc tcccaatcat
     3181 cctatggagg gggaaccatg atggttatcc ccatcttata aataaagcaa cagaggctta
     3241 gaaggacgaa ctctttttct caaggttacc cagatcattt tgcagaagtc cctagatttg
     3301 aatcatgctc ttgctttgag gttaaagaca caggggaagt cgaactcctt atctcctata
     3361 tcctgaatag ggaaaaccaa acattgtcaa gaggagagga agcctgagag ttggcatgaa
     3421 tcagagtgct gggcagtctg gagtcttccc cactgagttg atgatggaac agtagagtgg
     3481 ggcctgagcc ccgttagggc atgagctgct gaatgattca tgtgaacacc atgcacatgg
     3541 gagtgaggtt ttgagcagtg tgccacagga gcctaccctc aggccccacc ataaaatgta
     3601 gggccagtcc tacattttat caatgacttg cgtgaacaca gaaaatgtgg atacaaccaa
     3661 aaggtaacaa tccaattaaa aaatggacaa aaaacttgaa taaacatttc tcaaaagaag
     3721 atatacaaat gatcaaaaag catatgaaaa aatgctcaac atcgctaatt gtaagagaaa
     3781 tgcaaatcaa aactataatg agataccacc ttatattgat taggaagact gctataaaaa
     3841 tagtaaacaa acaaacaaac aaagtaagtc ttggggagga tgcagagaaa ttaaaatttt
     3901 tgtgcactgt tagtgggaat gtaaaatggt gcagctgtta cgggaaacgg tatgacagtt
     3961 cctcaaaaaa taaagaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta
     4021 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4081 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4141 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4261 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4321 ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4381 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4441 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4501 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4561 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4621 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4681 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4741 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4801 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4861 gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg
     4921 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     4981 aggagcgcac gaccccatgc atcgataaaa taaaagattt tatttagtct ccagaaaaag
     5041 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     5101 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     5161 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     5221 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     5281 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     5341 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     5401 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     5461 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     5521 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     5581 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     5641 ccgacttgtg gtctcgctgc cttgggaggg tctcctctga gtgattgact acccgtcagc
     5701 gggggtcttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
     5761 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5821 attggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6421 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6721 gaagtttgcg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7381 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7621 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7741 cgcacatttc cccgaaaagt gccac
//
