LOCUS       sequence-64228-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pWzl_neo_DEST_flag_SKIL
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccggatcca gaattcgcca ccatggacta caaagacgat gacgacaagc aattgacaag
       61 tttgtacaaa aaagttggca tggaaaacct ccagacaaat ttctccttgg ttcagggctc
      121 aactaaaaaa ctgaatggga tgggagatga tggcagcccc ccagcgaaaa aaatgataac
      181 ggacattcat gtaaatggaa aaacgataaa caaggtgcca acagttaaga aggaacactt
      241 ggatgactat ggagaagcac cagtggaaac tgatggagag catgttaagc gaacctgtac
      301 ttctgttcct gaaactttgc atttaaatcc cagtttgaaa cacacattgg cacaattcca
      361 tttaagtagt cagagctcgc tgggtggacc agcagcattt tctgctcggc attcccaaga
      421 aagcatgtcg cctactgtat ttctgcctct tccatcacct caggttcttc ctggcccatt
      481 gctcatccct tcagatagct ccacagaact cactcagact gtgttggaag gggaatctat
      541 ttcttgtttt caagttggag gagaaaagag actctgtttg ccccaagtct taaattctgt
      601 tctccgagaa tttacactcc agcaaataaa tacagtgtgt gatgaactgt acatatattg
      661 ttcaaggtgt acttcagacc agcttcatat cttaaaggta ctgggcatac ttccattcaa
      721 tgccccatcc tgtgggctga ttacattaac tgatgcacaa agattatgta atgctttatt
      781 gcggccacga acttttcctc aaaatggtag cgtacttcct gctaaaagct cattggccca
      841 gttaaaggaa actggcagtg cctttgaagt tgagcatgaa tgcctaggca aatgtcaggg
      901 tttatttgca ccccagtttt atgttcagcc tgatgctccg tgtattcaat gtctggagtg
      961 ttgtggaa
//
