LOCUS       sequence-64233-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Yamazaki T, Chen S, Yu Y, Yan B, Haertlein TC, Carrasco MA, Tapia 
            JC, Zhai B, Das R, Lalancette-Hebert M, Sharma A, Chandran S, 
            Sullivan G, Nishimura AL, Shaw CE, Gygi SP, Shneider NA, Maniatis T,
            Reed R
  TITLE     FUS-SMN protein interactions link the motor neuron diseases ALS and 
            SMA.
  JOURNAL   Cell Rep. 2012 Oct 25;2(4):799-806. doi: 
            10.1016/j.celrep.2012.08.025. Epub 2012 Sep 27.
  PUBMED    23022481
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2012.08.025"; journalName: "Cell Rep"; date: 
            "2012-10-25- 25"; volume: "2"; issue: "4"; pages: "799-806"
COMMENT     Sequence Label: 3X FLAG-FUS-WT
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     64..88
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             182..247
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             649..660
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gctttagcag agctctccta tcagtgatag agatctccct atcagtgata gagatcgtcg
       61 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      121 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttat
      181 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
      241 tgacaagctt ggtaccgagc tcggatccat ggcctcaaac gattataccc aacaagcaac
      301 ccaaagctat ggggcctacc ccacccagcc cgggcagggc tattcccagc agagcagtca
      361 gccctacgga cagcagagtt acagtggtta tagccagtcc acggacactt caggctatgg
      421 ccagagcagc tattcttctt atggccagag ccagaacaca ggctatggaa ctcagtcaac
      481 tccccaggga tatggctcga ctggcggcta tggcagtagc cagagctccc aatcgtctta
      541 cgggcagcag tcctcctatc ctggctatgg ccagcagcca gctcccagca gcacctcggg
      601 aagttacggt agcagttctc agagcagcag ctatgggcag ccccagagtg ggagctacag
      661 ccagcagcct agctatggtg gacagcagca aagctatgga cagcagcaaa gctataatcc
      721 ccctcagggc tatggacagc agaaccagta caacagcagc agtggtggtg gaggtggagg
      781 tggaggtgga ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg
      841 tggcgggtta tggcaatcaa gaaccagagt ggtggaggtg gcagcggtgg ctatggacag
      901 caggaccgtg
//
