LOCUS       sequence-64241-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_FNDC3B
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(293..317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(329..349)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        334..663
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      514..649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(673..692)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccggatccc agtgtggtgg tacgtaggaa ttcatggact acaaagacga tgacgacaag
       61 caattgacaa gtttgtacaa aaaaggtggc atgatgatga ccgaccaaat ccctctggaa
      121 ctgccaccat tgctgaacgg agaggtagcc atgatgcccc acttggtgaa tggagatgca
      181 gctcagcagg ttattctcgt tcaagttaat ccaggtgaga ctttcacaat aagagcagag
      241 gatggaacac ttcagtgcat tcaagatgaa gtggtgaaga gagcctgcga ttgcccaact
      301 ttcttgtaca aagtggtctc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      481 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      661 aaaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      721 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      781 gtcgatccgg accgccacat c
//
