LOCUS       sequence-64244-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_EIF5A2
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(551..575)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(587..607)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        592..924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      772..910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gccggatccc agtgtggtgg tacgtaggaa ttcatggact acaaagacga tgacgacaag
       61 caattgacaa gtttgtacaa aaaagttggc atggcagacg aaattgattt cactactgga
      121 gatgccgggg cttccagcac ttaccctatg cagtgctcgg ccttgcgcaa aaacggcttc
      181 gtggtgctca aaggacgacc atgcaaaata gtggagatgt caacttccaa aactggaaag
      241 catggtcatg ccaaggttca ccttgttgga attgatattt tcacgggcaa aaaatatgaa
      301 gatatttgtc cttctactca caacatggat gttccaaata ttaagagaaa tgattatcaa
      361 ctgatatgca ttcaagatgg ttacctttcc ctgctgacag aaactggtga agttcgtgag
      421 gatcttaaac tgccagaagg tgaactaggc aaagaaatag agggaaaata caatgcaggt
      481 gaagatgtac aggtgtctgt catgtgtgca atgagtgaag aatatgctgt agccataaaa
      541 ccctgcaaat acccaacttt cttgtacaaa gtggtctcga ccctgtggaa tgtgtgtcag
      601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggaggcttt
      901 tttggaggcc taggcttttg caaaaagctt accatgaccg a
//
