LOCUS       sequence-64251-e        3265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3265)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
FEATURES             Location/Qualifiers
     source          1..3265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(166..270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     338..356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             723..743
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      1659..2203
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2320..2337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(2570..3265,1..96,97..165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3193..3212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
       61 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      361 tcgtcttcaa gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga
      421 aaccggatgc tgcaattcag agcggcagca agtgggggac agcagaagac ctgaccgccg
      481 cagagtggat gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg
      541 ctgggtgggc taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca
      601 tgtgtgtgct gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagct
      661 aacaccgtgc gtgttgacaa ttttacctct ggcggtgata atggttgcag ctagcaataa
      721 ttttgtttaa ctttaagaag gaggatccaa atgaattcac tctataccgc tgaaggtgta
      781 atggataaac actcgctgtg gcagcgttat gtcccgctgg tgcgtcacga agcattgcgc
      841 ctgcaggttc gactgcccgc gagcgtggaa cttgacgatc tgctacaggc gggcggcatt
      901 gggttactta atgccgtcga acgctatgac gccctacaag gaacggcatt tacaacttac
      961 gcagtgcagc gtatccgtgg cgctatgctg gatgaacttc gcagccgtga ctgggtgccg
     1021 cgcagcgtgc gacgcaacgc gcgtgaagtg gcacaggcaa tagggcaact ggagcaggaa
     1081 cttggccgca acgccacgga aactgaggta gcggaacgtt tagggatcga tattgccgat
     1141 tatcgccaaa tgttgctcga caccaataac agccagctct tctcctacga tgagtggcgc
     1201 gaagagcacg gcgatagcat cgaactggtt actgatgatc atcagcgaga aaacccgcta
     1261 caacaactac tggacagtaa tctgcgccag cgggtgatgg aagccatcga aacgttgccg
     1321 gagcgcgaaa aactggtatt aaccctctat taccaggaag agctgaatct caaagagatt
     1381 ggcgcggtgc tggaggtcgg ggaatcgcgg gtcagtcagt tacacagcca ggctattaaa
     1441 cggttacgca ctaaactggg taagttataa tctagaggcg ccactcgaga gtcgaccaaa
     1501 gcccgccgaa aggcgggctt ttctgtgccg gcatgataag ctgtcaaaca tgagaattac
     1561 aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa
     1621 atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     1681 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     1741 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     1801 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     1861 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     1921 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     1981 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     2041 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     2101 caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     2161 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc
     2221 cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg
     2281 taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     2341 gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg
     2401 ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact
     2461 ccgctagggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3241 aacgttcttc ggggcgaaaa ctctc
//
