LOCUS       sequence-64263-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A
  TITLE     Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end 
            rule pathway.
  JOURNAL   J Biol Chem. 2008 Aug 29. 283(35):24011-28.
  PUBMED    18566452
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Aug 29. 283(35):24011-28."
COMMENT     Sequence Label: UPR pBARUra3
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..574
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             581..607
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             635..862
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 actgtacgaa ttcatggttc gaccattgaa ctgcatcgtc gccgtgtccc aaaatatggg
       61 gattggcaag aacggagacc taccctggcc tccgctcagg aacgagttca agtacttcca
      121 aagaatgacc acaacctctt cagtggaagg taaacagaat ctggtgatta tgggtaggaa
      181 aacctggttc tccattcctg agaagaatcg acctttaaag gacagaatta atatagttct
      241 cagtagagaa ctcaaagaac caccacgagg agctcatttt cttgccaaaa gtttggatga
      301 tgccttaaga cttattgaac aaccggaatt ggcaagtaaa gtagacatgg tttggatagt
      361 cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca gactctttgt
      421 gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg atttggggaa
      481 atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg aaaaaggcat
      541 caagtataag tttgaagtct acgagaagaa agacggtacc tacccatacg atgtcccaga
      601 ttacgccgct ttccttgcgg gtagtgggtc gaccatgcag attttcgtca agactttgac
      661 cggtaaaacc ataacattgg aagttgaatc ttccgatacc atcgacaacg ttaagtcgaa
      721 aattcaagac aaggaaggta tccctccaga tcaacaaaga ttgatctttg ccggtaggca
      781 gctagaagac ggtagaacgc tgtctgatta caacattcag aaggagtcca ccttacatct
      841 tgtgctaagg ctaagaggtg gtagacacgg atccggagct tggctgttgc ccgtctcact
      901 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      961 cgattc
//
