LOCUS       sequence-64273-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  TITLE     Mutations_Koga_Nature
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FR55
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(472..490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      486..533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      570..998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(657..676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     867..888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata tacatatggg cgaaatggat attcgttttc
      181 gcggtgatga cctggaagca ctggaaaaag ctctgaaaga aatgatccgt caggcgcgca
      241 aattcgccgg caccgtcacg tataccctgg atggtaacga cctggaaatt cgtatcaccg
      301 gcgtgccgga acaagttcgc aaagaactgg cgaaagaagc cgaacgtctg gcgaaagaat
      361 ttaatattac ggttacctac acgatccgcg gtagcctcga gcaccaccac caccaccact
      421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      541 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      781 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacg
//
