LOCUS       sequence-64309-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CAAA
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(618..636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(619..636)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(654..670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(654..670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(667..689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    678..694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(702..708,709..726,727..732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    747..768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(885..902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tacgactcnc tatagggcga attgggccct ctagatgcat gctcgagcgg ccgccagtgt
       61 gatggatatc tgcagaattc gcccttgccg tcctagcgcg tctcggctca ccccagagca
      121 ggtcgtggcg atcgcaagcc acgacggagg aaagcaagcc ttggaaacag tacagaggct
      181 gttgcctgtg ctgtgccaag cgcacggcct caccccagag caggtcgtgg caatcgcctc
      241 caacattggc gggaaacagg cactcgagac tgtccagcgc ctgcttcccg tgctgtgcca
      301 agcgcacggc ctcaccccag agcaggtcgt ggcaatcgcc tccaacattg gcgggaaaca
      361 ggcactcgag actgtccagc gcctgcttcc cgtgctgtgc caagcgcacg gattaacccc
      421 agagcaggtc gtggcaatcg cctccaacat tggcgggaaa caggcactcg agactgtcca
      481 gcgcctgctt cccgtgctgt gccaagcgca cggactcaga gacgtgggca caggaaagaa
      541 gggcgaattc cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg
      601 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      661 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      721 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      781 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      841 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaata
//
