LOCUS       sequence-64311-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..121)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(568..592)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(615..818)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(644..664)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttaacg cgccaccggt
       61 tagcgctagc tcattactac gtagaatcga gaccgaggag agggttaggg ataggcttac
      121 caaccacttt gtacaagaaa gttgggtatg atttttcatg tgaagattta ggtgtttctc
      181 catcattttc ctcttcctca tcctcttcac aatccccatc tgtttgctgt tccagttcct
      241 cttttgttat catttcaaaa tcatttccat ccagaaaaac ctgatcatca tgtggctcaa
      301 gtttgaattt tttcttagtt tccttctttt taggagttcc tggagtttcc tcctttttgg
      361 attcttcctt ttcaccatct ttttttttct ctttttcttt ttttgtcttc tcatccttta
      421 tattttcttt cttgctcttt ttatcttctt cttttccact ttcagctttt cctttatctt
      481 tttctttctt tatgtcttta tcatatttca ttttcattac ttttagggct cggtgaaaaa
      541 actgcttctt taaaagcctg ttgcacatgc caactttttt gtacaaactt gttgatcccg
      601 acagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      661 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      721 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      781 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      841 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc at
//
