LOCUS       sequence-64312-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CGTG
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(620..638)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(621..638)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(656..672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(656..672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(669..691)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    680..696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(704..710,711..728,729..734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    749..770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(887..904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatacgactc actatagggc gaattgggcc ctctagatgc atgctcgagc ggccgccagt
       61 gtgatggata tctgcagaat tcgcccttgc cgtcctagcg cgtctcggct caccccagag
      121 caggtcgtgg cgatcgcaag ccacgacgga ggaaagcaag ccttggaaac agtacagagg
      181 ctgttgcctg tgctgtgcca agcgcacgga ctcaccccag agcaggtcgt ggcaatcgcg
      241 agcaataacg gcggaaaaca ggctttggaa acggtgcaga ggctccttcc agtgctgtgc
      301 caagcgcacg gcctcacccc agagcaggtc gtggccattg cctcgaatgg agggggcaaa
      361 caggcgttgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca cggattaacc
      421 ccagagcagg tcgtggcaat cgcgagcaat aacggcggaa aacaggcttt ggaaacggtg
      481 cagaggctcc ttccagtgct gtgccaagcg cacggactca gagacgtggg cacaggaaag
      541 aagggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccaagct
      601 tgatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
      661 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      721 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      781 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      841 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      901 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      961 ggttatccac agaat
//
