LOCUS       sequence-64317-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GAAA
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(619..637)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(620..637)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(668..690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    679..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(703..709,710..727,728..733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    748..769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(886..903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtatacgact cnctataggg cgaattgggc cctctagatg catgctcgag cggccgccag
       61 tgtgatggat atctgcagaa ttcgcccttc tttcctgtgc ccacgtctct taagccgtgc
      121 gcttggcaca gcacgggaag caggcgctgg acagtctcga gtgcctgttt cccgccaatg
      181 ttggaggcga ttgccacgac ctgctctggg gttaatccgt gcgcttggca cagcacggga
      241 agcaggcgct ggacagtctc gagtgcctgt ttcccgccaa tgttggaggc gattgccacg
      301 acctgctctg gggtgaggcc gtgcgcttgg cacagcacgg gaagcaggcg ctggacagtc
      361 tcgagtgcct gtttcccgcc aatgttggag gcgattgcca cgacctgctc tggggtgagt
      421 ccgtgcgctt ggcacagcac tggaaggagc ctctgcaccg tttccaaagc ctgttttccg
      481 ccgttattgc tcgcgattgc cacgacctgc tctggggtca ggagacgcgc taggacggca
      541 agggcgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
      601 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      721 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      781 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      841 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaat
//
