LOCUS       sequence-64356-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM839
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg aaaatcgaag aaggaaaagt tgttatttgg catgcaatgc
      181 aacccaatga gcttgaggtc ttccaaagtt tagcggaaga atacatggca ctctctccag
      241 aagttgagat agtttttgaa caaaagccaa acttggaaga tgctcttaag gctgcaatac
      301 ccacaggtca aggtcctgac ctctttatct gggctcacga ctggattgga aagtttgctg
      361 aggcaggatt acttgagcca attgatgaat atgtaactga agatctcctt aacgagtttg
      421 ctccaatggc ccaggatgca atgcagtata aaggtcacta ctatgctcta ccattcgccg
      481 ctgaaacagt tgcaataatc tacaacaaag aaatggttag cgagccaccg aaaacctttg
      541 atgagatgaa ggcaataatg gagaagtact atgatccagc aaatgagaag tatggaatag
      601 cttggccaat taatgcctac tttatctcag caattgctca ggcctttggt ggttactact
      661 ttgacgacaa aacagagcaa ccgggactag ataagcctga gacaatcgaa ggatttaagt
      721 tcttcttcac agaaatatgg ccatacatgg ctccaactgg agactacaac actcaacaga
      781 gtatattcct c
//
