LOCUS       sequence-64357-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM839
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt gatcccttgc atgttgttaa ggatttcttg ttgatacttc
      301 tttagtattc cttcaatgtc agcgttttgt ggatcttgga ggatttcgtt aattgcccca
      361 tcaactccgc cccaaacagc actcatcttt ggactctttg gcattaggta tgcgtgctga
      421 actgcttgtc caaagccata gattactgga tcattcttaa tttctggatc atcaagaacc
      481 ttcgtaagaa ccggtatgta tcccagctct agtgccaatg tcttaattga ctcttcgctt
      541 gtggtaagcc actttgcgaa cttccatgca gcatccttgt tctttattcc cgctgcgaag
      601 taaatcaact taactccacc gtaaggcctt ggccagtact ccttaccatc cttgattatt
      661 ggaggtagtg gaaccactcc aaagtttatt cctgccttct taacgtcgtt aatgctccat
      721 ggaccattaa ccatcattgg ggctctaccc tcgaggaata tactctgttg agtgttgtag
      781 tctccagttg gagccatgta tgg
//
