LOCUS       sequence-64358-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM839
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(285..303)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      299..346
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(521..526,527..543,544..549))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(576..594)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        662..766
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgatcc
      241 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      301 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      361 tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
      421 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
      481 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata
      541 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      601 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
//
