LOCUS       sequence-64369-e       12387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Murbartian J, Arias JM, Lee JH, Gomora JC, Perez-Reyes E
  TITLE     Alternative splicing of the rat Ca(v)3.3 T-type calcium channel gene
            produces variants with distinct functional properties(1).
  JOURNAL   FEBS Lett. 2002 Sep 25;528(1-3):272-8.
  PUBMED    12297319
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "2002-09-25"; volume: "528(1-3)"; pages: "272-8"
COMMENT     Sequence Label: alpha-1Ic-HE2-pcDNA3
FEATURES             Location/Qualifiers
     source          1..12387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           962..1004
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(7939..7957)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7940..7957)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7977..7994)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7983..8207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8253..8681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8340..8359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8550..8571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8690..8710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8695..9025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8876..9011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9092..9886
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9146..9165)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9756..9775
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10060..10181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10097..10116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10151..10170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10230..10246)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10230..10246)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10243..10265)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10254..10270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10278..10284,10285..10302,10303..10308))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10323..10344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10461..10478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10632..11220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10712..10731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11391..12182,12183..12251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12014..12033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12252..12356)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctatcgatt
      901 gttctttttg cagaagctca gaataaaacg ctcaactttg gcaagcttgg tacccagctt
      961 gcttgttctt tttgcagaag ctcagaataa acgctcaact ttggcagatc aattccccgg
     1021 cttcggggac acgcgtcaac cccgcgtctc tgcccgggac gaccccgctg cccggccacg
     1081 tccatgccaa gggctccctg ctccacgctg acatggctga cagcaactta ccgccctcat
     1141 ctgcagcagc cccggcccct gagccgggaa tcactgagca gccggggccc cggagtcccc
     1201 ctccatcccc tccaggcctg gaggagccat tggaaggaac caaccctgac gtcccacatc
     1261 cagacctggc tcctgttgct ttcttctgcc tgcgccagac cacgagccca cggaactggt
     1321 gcatcaagat ggtttgtaac ccgtggttcg agtgtgtgag catgctggtt attctgctga
     1381 actgtgtgac cctgggcatg taccagccat gtgatgacat ggagtgcctg tcggaccgtt
     1441 gcaagatcct gcaggtcttc gatgacttca tcttcatctt ctttgccatg gagatggtgc
     1501 ttaagatggt ggccctgggc atttttggca agaagtgcta cctcggagac acatggaacc
     1561 gcctggattt cttcattgtc atggcaggga tggttgagta ctctctggac ctacagaaca
     1621 tcaacctgtc agccatccgc actgtgcgtg tcctgaggcc tctcaaagcc atcaaccgtg
     1681 tacccagcat gcggatcctg gtgaacctgc tgctcgacac gctgcccatg ctggggaacg
     1741 tgctcctgct ctgtttcttc gtcttcttca tcttcggcat cattggcgtg cagctctggg
     1801 caggcctgct acggaaccgc tgcttcctgg aagagaactt caccatacaa ggggatgtgg
     1861 ccctgccccc ttattaccaa ccagaggagg atgacgagat gccctttatc tgctccctga
     1921 ctggggacaa tggcatcatg ggctgccacg agatcccccc actgaaggag cagggccggg
     1981 aatgctgcct gtccaaagat gatgtgtatg acttcggggc ggggcgccag gacctcaacg
     2041 ccagcggtct gtgcgtcaac tggaaccgct actacaacgt ctgccgcacg ggcaacgcca
     2101 accctcacaa gggcgccatc aactttgaca acattggcta tgcctggatt gtgattttcc
     2161 aggtgatcac tctggaaggc tgggtggaga tcatgtacta tgtgatggac gcacattctt
     2221 tctacaactt catctacttc attctgctca tcatagtggg ctccttcttc atgatcaact
     2281 tgtgcctcgt tgtcatagca acccagttct ctgagaccaa gcaacgggag caccggctga
     2341 tgctggagca acgccagcgc tacctgtcct ccagcacggt ggccagttac gctgagcccg
     2401 gtgattgcta tgaggagatc ttccaatatg tctgtcacat ccttcgcaaa gccaagcgcc
     2461 gtgccctagg cctctaccag gccctgcaga accggcgcca ggccatgggc ccggggacac
     2521 cagcccctgc caagcctggg ccccatgcca aggagcccag ccactgcaag ctgtgcccac
     2581 gacacagccc cctggacccc actccccaca cactggtgca gcccatctct gccattctgg
     2641 cctctgaccc cagcagctgc cctcactgcc agcacgaggc aggcaggcgg ccctctggcc
     2701 tgggcagcac tgactcaggc caggaaggct caggttctgg tggctctgca gaggccgaag
     2761 ccaatgggga tggactccag agcagtgagg atggggtctc ctcggacctg gggaaggagg
     2821 aggaacagga ggacggggca gcccgactgt gtggggatgt gtggcgcgag acacgaaaaa
     2881 agctgcgggg catcgtggac agcaagtact tcaacagagg tatcatgatg gctatcctgg
     2941 tgaacacagt cagcatgggc atcgagcacc acgaacagcc cgaggagctg accaacatcc
     3001 tggagatctg caatgtggtc ttcaccagta tgtttgccct ggagatgatc ctgaaactgg
     3061 ccgcctttgg gctcttcgac tacctgcgga acccttacaa catctttgac agcatcatcg
     3121 tcatcatcag catctgggaa atcgtggggc aggcggacgg tggcctgtct gtgctgcgca
     3181 ccttccggtt gctgcgggtg ctgaagctgg tgcgcttcat gccggcgctg cggcgccagc
     3241 tcgtggtgct catgaagacc atggacaacg tggccacctt ctgcatgcta ctcatgctgt
     3301 tcatcttcat cttcagcatc cttgggatgc atatctttgg ctgcaaattc agcctccgca
     3361 cggacacggg agacaccgtt cctgacagga agaacttcga ttccttactg tgggccatcg
     3421 tcacagtgtt ccagatcctc actcaggagg actggaacgt tgtcctgtac aatggcatgg
     3481 cctccaccac cccctgggcc tccctctatt ttgttgccct catgaccttt ggcaactacg
     3541 ttctcttcaa tctcctggtg gctatcctgg tagagggttt ccaggctgag ggtgatgcta
     3601 atcgttcctg ctctgatgag gaccagagct catccaattt ggaggagttt gacaagctcc
     3661 cagagggcct ggacaacagt agagatctca agctctgccc aatacccatg acacccaatg
     3721 gacacctgga ccctagcctc cctctgggtg cgcatctggg tcctgctggt accatgggta
     3781 ctgccccccg cctctcactg cagccagacc cggtactggt ggccctagac tctcggaaaa
     3841 gcagtgtcat gtccctgggc aggatgagct atgatcagcg atccttgtcc agctcccgga
     3901 gctcctacta cgggccctgg ggccgcagtg ggacctgggc tagccgccgc tccagctgga
     3961 acagcctgaa acacaagccg ccctcagctg agcatgagtc cttactgtct ggggagggtg
     4021 gaggtagctg cgtcagggcc tgtgaaggcg cccgggagga ggcgccaact cgcaccgcac
     4081 ccctgcatgc tccacacgcg caccacgcgc accatggacc ccacctggca caccgtcacc
     4141 gacaccaccg ccggactctg tcccttgata ccagggactc tgttgacctg ggagagctgg
     4201 tgcccgtggt gggtgcccac tcacgggccg cttggagggg ggcgggtcag gcccctgggc
     4261 acgaggactg caatggcaga atgcccaaca tagccaagga tgtcttcacc aagatggatg
     4321 accgccgcga ccgcggggag gacgaggagg agatcgacta taccctgtgt ttccgggtcc
     4381 gcaagatgat tgatgtgtac aagccggact ggtgcgaagt ccgcgaggac tggtcggtct
     4441 acctcttctc ccccgagaac aagttccgga tcctgtgtca gaccatcatt gctcacaagc
     4501 tttttgacta cgtggtcttg gcctttatct tcctcaactg tatcaccatt gctctggaga
     4561 gaccccagat tgaagctggt agcactgagc gcatcttcct cacggtgtct aactacatct
     4621 tcacagccat cttcgtgggc gagatgacac tgaaggtggt ttctctgggc ctgtactttg
     4681 gtgagcaggc gtacctgcgt agcagctgga atgtactgga tggtttcctg gtctttgtgt
     4741 ccatcatcga tatcgtagtg tccgtggcct ctgctggggg agccaagatt ctgggggtcc
     4801 tccgggtcct gcggctcctg cgtaccttac gtcctttgag ggttatcagc cgggcccctg
     4861 ggctgaagct ggtggtagag acgctcatct cctccctcaa gcccattggg aacatcgtcc
     4921 tcatctgctg tgccttcttc atcatcttcg gcatcctggg ggtgcagctt ttcaaaggca
     4981 agttctacca ttgtttggga gtggacaccc gaaacatcac caaccgatct gactgcgtgg
     5041 cggccaacta ccgctgggtg catcacaaat acaactttga caacctgggc caggcattga
     5101 tgtccctctt tgtcttggcc tccaaggacg gctgggtgaa catcatgtat aatggattag
     5161 atgctgttgc tgtggaccag cagccagtga cgaaccacaa cccctggatg ctactgtact
     5221 tcatttcgtt cctgctcatc gtcagcttct ttgtgctcaa catgtttgtg ggcgtggtcg
     5281 tggagaactt ccacaagtgc cggcagcacc aggaggctga ggaggcgcgg aggcgtgagg
     5341 agaaacggct gcggcgcctg gaaaagaagc gccgtaaggc tcagaggctg ccctactatg
     5401 ctacctactg tcccacaagg ctgctcatcc actccatgtg caccagccac tacctggaca
     5461 tcttcattac cttcatcatc tgcctcaatg ttgtcaccat gtccctggag cactacaacc
     5521 agcctacatc cctagagaca gcccttaagt actgcaacta catgttcacc actgtctttg
     5581 tgctggaggc tgtgctgaag ctggtggcat ttggcctgag gcgtttcttc aaggaccgat
     5641 ggaaccagct ggacctggcc attgtgctgc tgtccgtcat gggcatcaca ctggaggaga
     5701 tcgagatcaa tgccgccctt cccatcaacc ccaccatcat ccgtatcatg cgtgttctgc
     5761 gtatcgcccg ggtgttgaag ctattgaaga tggccacagg aatgcgggcc ctgctggaca
     5821 cagtggtaca ggctctgccc caggtgggca acctgggcct gctcttcatg ctgctcttct
     5881 tcatctatgc tgctctggga gtggagctct tcggaaagct ggtctgcaat gacgagaacc
     5941 cgtgtgaggg catgagccgg cacgccacct ttgaaaactt cggcatggcc ttcctcacgc
     6001 tcttccaggt ctccacaggc gataactgga atggaattat gaaggacacc ctgcgagact
     6061 gtacccatga tgagcgcagc tgcctaagca gcctgcagtt tgtgtcaccg ctctactttg
     6121 tgagcttcgt gctcacagct cagttcgtgc tcatcaacgt ggtggtggcc gtgctgatga
     6181 aacatctgga tgacagcaac aaggaggccc aggaggatgc agagatggat gctgagatcg
     6241 agctggagat ggcccatggc ctcggcccct gccctggccc ctgccctggt ccctgcccct
     6301 gcccctgccc ctgcccctgt cctggcccga ggctgcccac tagttcacct ggggctccgg
     6361 ggcgaggatc gggaggggca ggtgctggag gcgacaccga gagtcacctg tgccggcact
     6421 gctattctcc agcccaggag accctgtggc tggacagcgt ctctttaatc atcaaggact
     6481 ccttggaggg ggagctgacc atcattgaca acctgtctgg gtccgtcttc caccactacg
     6541 cctcacctga cggctgtggc aagtgtcacc atgacaagca agagggacct gttgctcagc
     6601 gctccaggcc caggctgccc catcccagcc ccagggtgac ccgaggccac cacctccccc
     6661 tgcaggtgca gctggctgag acagaggcct tctccctgaa ctcagacagg tcttcatcca
     6721 tcctgctggg ggatgacctg agtcttgagg accccacggc ctgcccacag ggccccaagg
     6781 agagcaaggg tgaactagag cctccggagc ccatgcaggc tggagacctg gatgaatgct
     6841 ttttcccctt tgccagcgag ccagtgtcca caggcccaga gagcctgctg tgcgagatgg
     6901 gggccattcc attcaaccct gtccagtcct ggctcaaaca cgagagcagc caagcacccc
     6961 agagcccttt ctccccggat ggctccagcc ctctcctgca gatgcctgct gagttcttcc
     7021 accctgctgt gtctgccagc cagaaggggc aggaaccggg catgagtgca ggaaccctgc
     7081 ccaagattgc acttcagggg tcctgggcat cgctgaggtc accgagtgtc aactgcaccc
     7141 tcttgcgcca ggctactgtg agtgacacgt ccttggatgc cagtcctagc agctcagcgg
     7201 gcagcctaca gaccacactg gaagacagtc tgactctgag tgacagtccc cggcgtgccc
     7261 tggggccgcc ggtccaggtg cctgggccac gggctagcct gtcaccggcc acccggcgcc
     7321 gcctcagcct gcggggccgt ggcctgttta gtctgcgtgg gctgcgggcc catcagcgta
     7381 gccacagcag tggcggctcc accagccctg gctgcactca ccacgactcc atggacccct
     7441 ctgatgagga gggccgcggg ggcgcaggtg gcgggggtgc aggcagtgag cactcggaga
     7501 ccctcagcag cctgtcactc acatctctct tctgcctacc acccacgctg ccaccacccg
     7561 gcctcacccc agccaggaag ttcaacagta ccagcagcct ggccgctgga cctggccgtc
     7621 ctggctccac tgtctcagcc cgtggtctgg tcagaagccc ctcatgggct gcagaccgca
     7681 gcaaggaccc accaggccaa gctcagctgg tctcaggctt gggctcctca gcacccgggc
     7741 cacagccacc tcccggggag tcaactgatg ctgcgagcaa aaggaagaga tgaggggccg
     7801 caggggctgc gggggacctg ctacccgcta tcctcccgcc tgccccatct tgccttcttt
     7861 tacctcagga gccagggaga attctgcaga tatccatcac actggcggcc gctcgagcat
     7921 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     7981 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8041 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8101 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8161 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8221 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     8281 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8341 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8401 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8461 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8521 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8581 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     8641 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8701 cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca
     8761 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8821 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8881 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8941 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9001 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9061 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     9121 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9181 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9241 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     9301 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     9361 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     9421 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     9481 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     9541 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     9601 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     9661 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     9721 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9781 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     9841 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     9901 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     9961 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10021 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10141 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    10201 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10261 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10321 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10381 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10441 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10801 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
    10861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    11101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    11161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    11221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    11281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    11341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    11401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    11461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11701 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    11821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12001 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    12181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    12241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    12301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    12361 tttccccgaa aagtgccacc tgacgtc
//
