LOCUS       sequence-64371-e       13225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13225)
  AUTHORS   Gomora JC, Murbartian J, Arias JM, Lee JH, Perez-Reyes E
  TITLE     Cloning and expression of the human T-type channel Ca(v)3.3: 
            insights into prepulse facilitation.
  JOURNAL   Biophys J. 2002 Jul;83(1):229-41.
  PUBMED    12080115
REFERENCE   2  (bases 1 to 13225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2002-07"; volume: "83(1)"; pages: "229-41"
COMMENT     Sequence Label: a1Ic-HE3-pcDNA3
FEATURES             Location/Qualifiers
     source          1..13225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7756..7765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8777..8795)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8778..8795)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8815..8832)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8821..9045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9091..9519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9178..9197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9388..9409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9528..9548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9533..9863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9714..9849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9776..9795
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9930..10724
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9984..10003)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10594..10613
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10898..11019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10935..10954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10989..11008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11068..11084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11068..11084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11081..11103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11092..11108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11116..11122,11123..11140,11141..11146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11161..11182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11299..11316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11470..12058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11550..11569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12229..13020,13021..13089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12852..12871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13090..13194)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctatcgatt
      901 gttctttttg cagaagctca gaataaaacg ctcaactttg gcaagcttgg taccgagctc
      961 ggatccacta gtaacggccg ccagtgtgct ggaattcggc ttgctcagtg tggacatggc
     1021 tgagagcgcc tccccgccct cctcatctgc agcagcccca gccgctgagc caggagtcac
     1081 cacggagcag cccggacccc ggagcccccc atcttccccg ccaggcctgg aggagcctct
     1141 ggatggagct gatcctcatg tcccacaccc agacctggcg cctattgcct tcttctgcct
     1201 gcgacagacc accagccccc ggaactggtg catcaagatg gtgtgcaacc cgtggtttga
     1261 atgtgtcagc atgctggtga tcctgctgaa ctgcgtgaca cttggcatgt accagccgtg
     1321 cgacgacatg gactgcctgt ccgaccgctg caagatcctg caggtctttg atgacttcat
     1381 ctttatcttc tttgccatgg agatggtgct caagatggtg gccctgggga tttttggcaa
     1441 gaagtgctac ctcggggaca catggaaccg cctggatttc ttcatcgtca tggcagggat
     1501 ggtcgagtac tccctggacc ttcagaacat caacctgtca gccatccgca ccgtgcgcgt
     1561 cctgaggccc ctcaaagcca tcaaccgcgt gcccagtatg cggatcctgg tgaacctgct
     1621 cctggacaca ctgcccatgc tggggaatgt cctgctgctc tgcttctttg tcttcttcat
     1681 ctttggcatc ataggtgtgc agctctgggc gggcctgctg cgtaaccgct gcttcctgga
     1741 ggagaacttc accatacaag gggatgtggc cttgccccca tactaccagc cggaggagga
     1801 tgatgagatg cccttcatct gctccctgtc gggcgacaat gggataatgg gctgccatga
     1861 gatccccccg ctcaaggagc agggccgtga gtgctgcctg tccaaggacg acgtctacga
     1921 ctttggggcg gggcgccagg acctcaatgc cagcggcctc tgtgtcaact ggaaccgtta
     1981 ctacaatgtg tgccgcacgg gcagcgccaa cccccacaag ggtgccatca actttgacaa
     2041 catcggttat gcttggattg tcatcttcca ggtgatcact ctggaaggct gggtggagat
     2101 catgtactac gtgatggatg ctcactcctt ctacaacttc atctacttca tcctgcttat
     2161 catagtgggc tccttcttca tgatcaacct gtgcctcgtt gtcatagcga cccagttctc
     2221 ggagaccaag caacgggagc accggctgat gctggagcag cggcagcgct acctgtcctc
     2281 cagcacggtg gccagctacg ccgagcctgg cgactgctac gaggagatct tccagtatgt
     2341 ctgccacatc ctgcgcaagg ccaagcgccg cgccctgggc ctctaccagg ccctgcagag
     2401 ccggcgccag gccctgggcc cggaggcccc ggcccccgcc aaacctgggc cccacgccaa
     2461 ggagccccgg cactaccagc tgtgcccgca acatagcccc ctggatgcga cgccccacac
     2521 cctggtgcag cccatccccg ccacgctggc ttccgatccc gccagctgcc cttgctgcca
     2581 gcatgaggac ggccggcggc cctcgggcct gggcagcacc gactcgggcc aggagggctc
     2641 gggctccggg agctccgctg gtggcgagga cgaggcggat ggggacgggg cccggagcag
     2701 cgaggacgga gcctcctcag aactggggaa ggaggaggag gaggaggagc aggcggatgg
     2761 ggcggtctgg ctgtgcgggg atgtgtggcg ggagacgcga gccaagctgc gcggcatcgt
     2821 ggacagcaag tacttcaacc ggggcatcat gatggccatc ctggtcaaca ccgtcagcat
     2881 gggcatcgag caccacgagc agccggagga gctgaccaac atcctggaga tctgcaatgt
     2941 ggtcttcacc agcatgtttg ccctggagat gatcctgaag ctggctgcat ttgggctctt
     3001 cgactacctg cgtaacccct acaacatctt cgacagcatc attgtcatca tcagcatctg
     3061 ggagatcgtg gggcaggcgg acggtgggct gtcggtgctg cggaccttcc ggctgctgcg
     3121 cgtgctgaaa ctggtgcgct tcatgcctgc cctgcggcgc cagctcgtgg tgctcatgaa
     3181 gaccatggac aacgtggcca ccttctgcat gctgctcatg ctcttcatct tcatcttcag
     3241 catccttggg atgcatattt ttggctgcaa gttcagcctc cgcacggaca ctggagacac
     3301 ggtgcccgac aggaagaact tcgactccct gctgtgggcc atcgtcactg tgttccagat
     3361 cctcacccag gaggactgga acgtcgttct ctacaatggc atggcctcca cttctccctg
     3421 ggcctccctc tactttgtcg ccctcatgac cttcggcaac tatgtgctct tcaacctgct
     3481 ggtggccatc ctggtggagg gcttccaggc ggagggtgac gccaatcgct cctactcgga
     3541 cgaggaccag agctcatcca acatagaaga gtttgataag ctccaggaag gcctggacag
     3601 cagcggagat cccaagctct gcccaatccc catgaccccc aatgggcacc tggaccccag
     3661 tctcccactg ggtgggcacc taggtcctgc tggggctgcg ggacctgccc cccgactctc
     3721 actgcagccg gaccccatgc tggtggccct gggctcccga aagagcagcg tcatgtctct
     3781 agggaggatg agctatgacc agcgctccct gtccagctcc cggagctcct actacgggcc
     3841 atggggccgc agcgcggcct gggccagccg tcgctccagc tggaacagcc tcaagcacaa
     3901 gccgccgtcg gcggagcatg agtccctgct ctctgcggag cgcggcggcg gcgcccgggt
     3961 ctgcgaggtt gccgcggacg aggggccgcc gcgggccgca cccctgcaca ccccacacgc
     4021 ccaccacgtt catcacgggc cccatctggc gcaccgccac cgccaccacc gccggacgct
     4081 gtccctcgac aacagggact cggtggacct ggccgagctg gtgcccgcgg tgggcgccca
     4141 cccccgggcc gcctggaggg cggcaggccc ggcccccggg catgaggact gcaatggcag
     4201 gatgcccagc atcgccaaag acgtcttcac caagatgggc gaccgcgggg atcgcgggga
     4261 ggatgaggag gaaatcgact acaccctgtg cttccgcgtc cgcaagatga tcgacgtcta
     4321 taagcccgac tggtgcgagg tccgcgaaga ctggtctgtc tacctcttct ctcccgagaa
     4381 caggttccgg gtcctgtgtc agaccattat tgcccacaaa ctcttcgact acgtcgtcct
     4441 ggccttcatc tttctcaact gcatcaccat cgccctggag cggcctcaga tcgaggccgg
     4501 cagcaccgaa cgcatctttc tcaccgtgtc caactacatc ttcacggcca tcttcgtggg
     4561 cgagatgaca ttgaaggtag tctcgctggg cctgtacttc ggcgagcagg cgtacctacg
     4621 cagcagctgg aacgtgctgg atggctttct tgtcttcgtg tccatcatcg acatcgtggt
     4681 gtccctggcc tcagccgggg gagccaagat cttgggggtc ctccgagtct tgcggctcct
     4741 gcgcacccta cgccccctgc gtgtcatcag ccgggcgccg ggcctgaagc tggtggtgga
     4801 gacactcatc tcctccctca agcccatcgg caacatcgtg ctcatctgct gtgccttctt
     4861 catcatcttt ggcatcctgg gagtgcagct cttcaagggc aagttctacc actgtctggg
     4921 cgtggacacc cgcaacatca ccaaccgctc ggactgcatg gccgccaact accgctgggt
     4981 ccatcacaaa tacaacttcg acaacctggg ccaggctctg atgtccctct ttgtcctggc
     5041 atccaaggat ggttgggtga acatcatgta caatggactg gatgctgttg ctgtggacca
     5101 gcagcctgtg accaaccaca acccctggat gctgctgtac ttcatctcct tcctgctcat
     5161 cgtcagcttc tttgtgctca acatgtttgt gggtgtcgtg gtggagaact tccacaagtg
     5221 ccggcagcac caggaggctg aagaggcacg gcggcgtgag gagaagcggc tgcggcgcct
     5281 ggagaagaag cgccggaagg cccagcggct gccctactat gccacctatt gtcacacccg
     5341 gctgctcatc cactccatgt gcaccagcca ctacctggac atcttcatca ccttcatcat
     5401 ctgcctcaac gtggtcacca tgtccctgga gcactacaat cagcccacgt ccctggagac
     5461 agccctcaag tactgcaact atatgttcac cactgtcttt gtgctggagg ctgtgctgaa
     5521 gctggtggca tttggtctga ggcgcttctt caaggaccga tggaaccagc tggacctggc
     5581 cattgtgcta ctgtcagtca tgggcatcac cctggaggag atcgagatca atgcggccct
     5641 gcccatcaat cccaccatca tccgcatcat gagggttctg cgcattgccc gagtgctgaa
     5701 gctgttgaag atggccacag gaatgcgggc cctgctggac acggtggtgc aagctttgcc
     5761 ccaggtgggc aacctgggcc tcctcttcat gctgctcttc ttcatctatg ctgctctcgg
     5821 ggtggagctc tttgggaagc tggtctgcaa cgacgagaac ccgtgcgagg gcatgagccg
     5881 gcatgccacc ttcgagaact tcggcatggc cttcctcaca ctcttccagg tctccacggg
     5941 tgacaactgg aacgggatca tgaaggacac gctgcgggac tgcacccacg acgagcgcag
     6001 ctgcctgagc agcctgcagt ttgtgtcgcc gctgtacttc gtgagcttcg tgctcaccgc
     6061 gcagttcgtg ctcatcaacg tggtggtggc tgtgctcatg aagcacctgg acgacagcaa
     6121 caaggaggcg caggaggacg ccgagatgga tgccgagctc gagctggaga tggcccatgg
     6181 cctgggccct ggcccgaggc tgcctaccgg ctccccgggc gcccctggcc gagggccggg
     6241 aggggcgggc ggcgggggcg acaccgaggg cggcttgtgc cggcgctgct actcgcctgc
     6301 ccaggagaac ctgtggctgg acagcgtctc tttaatcatc aaggactcct tggaggggga
     6361 gctgaccatc atcgacaacc tgtcgggctc catcttccac cactactcct cgcctgccgg
     6421 ctgcaagaag tgtcaccacg acaagcaaga ggtgcagctg gctgagacgg aggccttctc
     6481 cctgaactca gacaggtcct cgtccatcct gctgggtgac gacctgagtc tcgaggaccc
     6541 cacagcctgc ccacctggcc gcaaagacag caagggtgag ctggacccac ctgagcccat
     6601 gcgtgtggga gacctgggcg aatgcttctt ccccttgtcc tctacggccg tctcgccgga
     6661 tccagagaac ttcctgtgtg agatggagga gatcccattc aaccctgtcc ggtcctggct
     6721 gaaacatgac agcagtcaag cacccccaag tcccttctcc ccggatgcct ccagccctct
     6781 cctgcccatg ccagccgagt tcttccaccc tgcagtgtct gccagccaga aaggcccaga
     6841 aaagggcact ggcactggaa ccctccccaa gattgcgctg cagggctcct gggcatctct
     6901 gcggtcacca agggtcaact gtaccctcct ccggcaggcc accgggagcg acacgtcgct
     6961 ggacgccagc cccagcagct ccgcgggcag cctgcagacc acgctcgagg acagcctgac
     7021 cctgagcgac agcccccggc gtgccctggg gccgcccgcg cctgctccag gaccccgggc
     7081 cggcctgtcc cccgccgctc gccgccgcct gagcctgcgc ggccggggcc tcttcagcct
     7141 gcgggggctg cgggcgcatc agcgcagcca cagcagcggg ggctccacca gcccgggctg
     7201 cacccaccac gactccatgg acccctcgga cgaggagggc cgcggtggcg cgggcggcgg
     7261 gggcgcgggc agcgagcact cggagaccct cagcagcctc tcgctcacct ccctcttctg
     7321 cccgccgccc ccgccgccag cccccggcct cacgcccgcc aggaagttca gcagcaccag
     7381 cagcctggcc gcccccggcc gcccccacgc cgccgccctg gcccacggcc tggcccggag
     7441 cccctcgtgg gccgcggacc gcagcaagga cccccccggc cgggcaccgc tgcccatggg
     7501 cctgggcccc ttggcgcccc cgccgcaacc gctccccgga gagctggagc cgggagacgc
     7561 cgccagcaag aggaagagat gagggtcgca ggggcccccg gccgcccacc gcccgccccg
     7621 tctcaccttc tttacctcag gagccaggag cagacagcaa tacttcgtcc acacctggga
     7681 tcgcgcaggg cccgcagggc acaggcgccc gacagccggg ctgagcggag tctgggttag
     7741 ccaggcctgc gtggcccatg gtggcccttc cagtgcatat acatacatat atatatatat
     7801 gcatatatat atatatatat atatatatat atatgtgtat acacacacac atagacagac
     7861 atatatatat atatttattt tttttactga gagcttatga cttccagaaa gtgctaaagg
     7921 tgggaggtgg gccagggcct ggacggggct tttgtctgat gctctgggat tctggccaga
     7981 cccaccccag ggcacatgtc ctgcgggggc gtcccagctg tgttttttga tgtctcctcc
     8041 ctggttaaga gtagcttgga gaggaccctc aggcctctga gggcaccagg ccctggagaa
     8101 gaggtgcaat tcagggtgtg tgtgtgtttt ttttccttta aagaagaaac gctgctaaga
     8161 tcccacgtgg ctcccacgtg tcggggtgtc tgtcctgtca tcctgactgt ctcgttattg
     8221 tgaagtcttt cgtagacacc ccagagcaca cacatcccct tagtccaccg gttagatgtc
     8281 tctctttaga aaaatcaggg gtgagtagct gtgtttcctt aggcctgggg taggggatgg
     8341 agagcagctc caaggctgag ctggggctct ggccccaggt gaggtcccca gctcctgagc
     8401 acttctgagg gggtgggttc cacccccagg agggcggggg tgggtggagc aggagtggag
     8461 gcagcctgca gaggaagggt gcgggagact gagccggcag tggcttgctt ggaggggcta
     8521 gggtacccgc ctggtctcgg ctggctccag ctgccctgca ggtgcccctg ggctcaggta
     8581 gttagttgtt cagccactaa tgccttttat cccccatatg ggcgctgagc tgtggctgtt
     8641 cctggacact gtgctgtccc cgtcctgagc aactatgccc ccgccccagt aggttcaagg
     8701 caaagcagct ctgaccgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
     8761 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     8821 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8881 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8941 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     9001 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     9061 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     9121 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9181 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9241 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9301 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9361 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9421 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
     9481 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     9541 gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc
     9601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9781 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     9841 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     9901 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     9961 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
    10021 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
    10081 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
    10141 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
    10201 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
    10261 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
    10321 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
    10381 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
    10441 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
    10501 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
    10561 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
    10621 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
    10681 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
    10741 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
    10801 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10861 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    11041 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    11101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    11161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    11221 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    11281 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    11341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    11401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    11461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11641 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
    11701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11881 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    12181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    12241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    12301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    12361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    12421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    12481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    12541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    12601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    12661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    13021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    13081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    13201 tccccgaaaa gtgccacctg acgtc
//
