LOCUS       sequence-64384-e       12298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Yang N, Zuchero JB, Ahlenius H, Marro S, Ng YH, Vierbuchen T, 
            Hawkins JS, Geissler R, Barres BA, Wernig M
  TITLE     Generation of oligodendroglial cells by direct lineage conversion.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):434-9. doi: 10.1038/nbt.2564. Epub 
            2013 Apr 14.
  PUBMED    23584610
REFERENCE   2  (bases 1 to 12298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2564"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "434-9"
COMMENT     Sequence Label: Tet-O-FUW-Zfp536
FEATURES             Location/Qualifiers
     source          1..12298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3945..4533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3998..4018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4416..4427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4536..4552)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4537..4553)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5058..5238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5264..5281)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5270..5494
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5540..5968
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5627..5646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5837..5858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5977..5997)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5982..6311
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6162..6297
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6224..6243
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6359..6406
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6425..6799
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6929..7050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6966..6985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7020..7039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7099..7115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7123..7139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7147..7153,7154..7171,7172..7177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7330..7347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7501..8089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7581..7600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8260..9051,9052..9120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8883..8902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9300..9319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9491..9870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9872..10070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10025..10045
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10088..10268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10315..10440
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10933..11166
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11351..11395
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11693..11810
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(11864..11882,11883..11905,11906..11924,11925..11947,
                     11948..11966,11967..11989,11990..12008,12009..12031,
                     12032..12050,12051..12073,12074..12092,12093..12115,
                     12116..12134)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        12167..12204
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12201..12225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcgccac catggaagaa gcgagtctgt gccttggagt gtcctcgacg gcaccggaag
       61 ctgagcctca tctgagcggc ccagtcctca atggccagta tgccatgagc cagaagttgc
      121 accagatcac ttcccagctc agccatgcct ttcctgagtt gcacccgcgg cccaacccag
      181 aggagaagac acctgcggcc cttgaggaga aggcccacgt gcccatgagc ggacagtcca
      241 tgggcagcca aatggcactg ctggccaacc agctgggccg tgatgtagac aacagcctca
      301 atgggcgtgt ggatctgcag cagttcctca atgggcagaa cctaggaatc atgtctcaga
      361 tgagtgacat tgaggatgat gcccgaaaga accggaagta tccgtgtccc ctgtgtggca
      421 agcgtttccg gttcaatagc atcctctccc tgcacatgcg cactcacacc ggtgagaagc
      481 cattcaagtg cccctactgc gaccaccgcg cggcccaaaa aggaaacctc aagatccacc
      541 tgcggaccca caagctgggc aacttgggga aggggcgcgg gcgggtacgt gaggagaacc
      601 gtctgctgca cgagctggag gagagggcca tcctgcggga caagcaaatg aagggcagcc
      661 tgctgcagcc aaggtctgat ctcaagccct tggcacatgc ccagcaggcc ccgttggcca
      721 cctgcaactt ggccttgcct cccaaccata gcgtgcccga tgtagcccac ccggcacctt
      781 cccccaagcc ggccaatttg caggaggatt cggtgacccc ggctgcgggc ttccgatgca
      841 ccttttgcaa aggcaaattc aagaagcgag aagagctgga tcgccacatc cgcattttgc
      901 ataagcccta caagtgcaca ctgtgtgact ttgccgcctc ccaggaggag gagctcatca
      961 gtcacgtgga gaaggcccat atcactgcgg agtctgccca gggccagggc cccaatggtg
     1021 ggggagagca gtcagcaaac gagttccgct gtgaggtatg tggccaggtg ttcagccagg
     1081 cctggttcct gaagggccac atgcgcaagc acaaagactc ttttgagcac tgctgccaga
     1141 tatgtgggcg gcgcttcaaa gagccttggt tcctgaagaa tcacatgaag gtccacctca
     1201 acaagctatc ggtgaagaac aaatccccca ctgagcctga ggtggcggtg cccatgggtg
     1261 gcctgtccca ggaggcccat gccaacctat actctaggta cctctcctgc ctgcagagtg
     1321 gctttatggc cccagacaaa gccagtctga atgagccctc tcagctctat ggcaaggggg
     1381 agctcccggc caaggagaag gaggttctgg gaaagctact gtctcccatt tccagtatgg
     1441 ctcacagtgt ccccgagggg gacaagcact ccctcctggg ctgcctcaac ctcgtgccac
     1501 cactgaaatc cagctgcatc gagcggttgc aggcagctgc caaagctgca gaaatggacc
     1561 ctgtgaatag ctatcaggcc tggcagctca tggccagggg catggccatg gaacacggct
     1621 tcttatctaa agagcatcag ctctcgcgca accacgaaga ccctttggca aacaccggag
     1681 ttctgtttga taaggagaag cgggagtacg tgttagtggg agcagatggc tccaagcaga
     1741 aaatgcctgc tgatttggtt cacagcacta aagtgggcaa tcagagagac ctgccaaata
     1801 agctcgaccc tctagaaggc agtcgggaat ttttgtcaca tgggctcaac cagacgctag
     1861 actacaacct gcagggtcct gggaacatga aagagaaacc cactgagtgc ccggattgtg
     1921 gacgggtttt ccgaacctac caccaggtgg tcgtgcattc tcgagtccac aagcgggacc
     1981 gcaagagcga tgaggacgct ctgcacgtgg gtgtgggcct ggaggagagg cgaggctcag
     2041 gcagcgacca ggagtcccag tcagtgagcc gctccaccac acctggctcc tctaacgtga
     2101 ctgaggagag tggggccgga ggtggcctgt cgcagaccgg aagcgcccaa gaggatagtc
     2161 cacacccctc ttcaccatct tcctcagaca tcggcgagga ggccgggaga gccggtggcg
     2221 tccagcagca ggcgctgctt cgagacagga acctgggctc ggccatgaag gactgcccgt
     2281 actgtgggaa gactttccgg acttcccatc accttaaggt ccacctgagg atacacacag
     2341 gtgagaagcc ctataagtgt cctcactgtg actatgctgg cacccagtca gcgtccctga
     2401 agtatcacct ggagagacac catcgggagc ggcagaatgg agccgggccc ctgtcaggac
     2461 agcccccgaa ccaggagcac aaggatgaga cctctagcaa agcgcctatg ttcatcaggc
     2521 cggacatcct tcgaggggct tttaagggtc tccccggaat cgacttccga ggtggtcccg
     2581 cctctcagca gtggacagcc ggcatgctct cctccggaga tcattcaggg caggccactg
     2641 gcatgccctc agagctgtct tcagatgccc taaaaggctc cgaccttcct tccaaaagct
     2701 cccactactc ggagatcgga agagcttatc aaaacatcgt gagcaacggt gtgaatttcc
     2761 aagggtccct gcaggccttc atggacagct ttgtcctcag ctctttgaag aaaaaggaca
     2821 caaaggacaa ggtcccgtct gatgcccatc ccatgaaagc ccatactgca gagggtggag
     2881 aggagaaagc aagcatgaag ccctcccagc gaaagtctga gaagtctcag tacgaacctc
     2941 tggacttgtc cgtgcggcca gacgccccca ctctccctgg ctcctccgtg accgtgcaag
     3001 acagcatcgc gtggcacggc tgcttgttct gcgctttcac cacctcgtcc atggagctga
     3061 tggcccttca cctccaggcc aaccacctag gcagagcaaa acgcaaagat caccccacag
     3121 gggtgacagt caactgcaaa gagcaggggc gggaggccag caaggtctct gtactgcctt
     3181 ccctacagtc aaacaaagag atggcccttc ctagcgctgt tggtgtgctc gactctgctc
     3241 ctgagaagtt ggcccaaggg ccagccaaag agactctagg ggacccgaag agtggtcagt
     3301 ggcccaacca catggaccct gccttttgta cctttccatc tgacttctac aaacagtttg
     3361 gcgtttaccc cgctatggtt ggctcagggg ccccgggctc ctgcctcaac aagaacaccg
     3421 aggggaaaac ccacccagat gacgacgccc ctatcctgat cccggaaacc acgaacaaaa
     3481 acactactga tgacctctca gatattgcct cctccgagga catggactcc tccaagggag
     3541 aaaacaatga ggacgaggag ctagacaccg aaccagaaat gacgagcaag cctctgtctg
     3601 ctctcagcaa ggatggcagc agtgagggcg gcgacagcct gctgtcccca ggcgccccgc
     3661 agcccatcca gggactggtc tcacctttag cccaagcagc cgaggaacag tggcacagcc
     3721 caggcctcct tcctgcccag gacccctcgg cggggctgcc caaaccagag cggggtcccc
     3781 cgggcttgga gaaacctatg agcatgctgt cggtcctcag ggcctacagc gccgatggct
     3841 tagcagcctt taacgggctt gctagcagca cagcaaattc tggatgtatc aagaggccag
     3901 acttgtgtgg taagttttag gaattcgata tcaagcttat cgataatcaa cctctggatt
     3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4081 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4141 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4201 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4261 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4321 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4381 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4441 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4501 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     4561 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     4621 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     4681 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4741 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     4801 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     4861 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     4921 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     4981 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     5041 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5221 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5281 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5341 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5401 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5461 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5521 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5761 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5821 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5881 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5941 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6001 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6061 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6241 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6301 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6361 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     6421 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     6481 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     6541 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     6601 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     6661 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     6721 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     6781 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     6841 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6901 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7021 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9301 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9361 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9421 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     9481 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9541 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     9841 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     9901 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     9961 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
    10021 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
    10081 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10141 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10201 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10261 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    10321 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    10381 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    10441 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    10501 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    10561 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10621 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10681 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10741 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
    10801 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    10861 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    10921 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    10981 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    11041 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    11101 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    11161 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    11221 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    11281 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    11341 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    11401 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    11461 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    11521 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    11581 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    11641 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
    11701 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    11761 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    11821 acagggacag cagagatcca gtttggttac tcgagtttac cactccctat cagtgataga
    11881 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
    11941 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
    12001 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
    12061 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
    12121 atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgagtag gcgtgtacgg
    12181 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
    12241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccg
//
