LOCUS       sequence-64385-e        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Yang N, Zuchero JB, Ahlenius H, Marro S, Ng YH, Vierbuchen T, 
            Hawkins JS, Geissler R, Barres BA, Wernig M
  TITLE     Generation of oligodendroglial cells by direct lineage conversion.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):434-9. doi: 10.1038/nbt.2564. Epub 
            2013 Apr 14.
  PUBMED    23584610
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2564"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "434-9"
COMMENT     Sequence Label: Tet-O-FUW-Olig2
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1008..1596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1061..1081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1479..1490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1599..1615)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1600..1616)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2121..2301
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2327..2344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2333..2557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2603..3031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2690..2709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2900..2921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3040..3060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3045..3374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3225..3360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3287..3306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3422..3469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3488..3862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3992..4113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4029..4048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4083..4102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4162..4178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4162..4178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4175..4197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4186..4202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4210..4216,4217..4234,4235..4240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4255..4276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4393..4410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4564..5152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4644..4663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5323..6114,6115..6183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5946..5965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6184..6288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6363..6382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6554..6933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6935..7133
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7088..7108
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7151..7331
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7378..7503
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7996..8229
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8414..8458
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8756..8873
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8927..8945,8946..8968,8969..8987,8988..9010,9011..9029,
                     9030..9052,9053..9071,9072..9094,9095..9113,9114..9136,
                     9137..9155,9156..9178,9179..9197)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9230..9267
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9264..9288
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcgccac catggactcg gacgccagcc tggtgtctag tcgcccatcg tccccagaac
       61 ccgatgatct ttttctgccc gcccggagca aggggggaag cagcagcggc ttcacaggag
      121 ggactgtgtc ctcgtccacg ccgagcgact gcccgccaga gctgagctcc gagctacgag
      181 gggccatggg cgcgtcaggc gcgcatcccg gggacaaact gggcggcggt ggcttcaagt
      241 catcttcctc cagcacctcc tcgtccacgt cctcggcggc cacgtcttcc accaagaaag
      301 acaagaagca gatgactgag cccgagctgc agcagctgcg cctgaagatc aacagccgcg
      361 aacgcaagcg catgcacgac ctcaacatcg ccatggacgg cctgcgggag gtcatgcctt
      421 acgcgcacgg gccgtcggtg cgcaagctct ccaagatcgc cacgctgctg ctggcgcgaa
      481 actacatcct catgctcacc aactcgctgg aggagatgaa gcgactggtg agcgagatct
      541 acgggggtca ccacgccggc ttccacccat cggcctgtgg cgggctggca cactcggcgc
      601 ctctacccac cgccacggcg caccccgcgg ccgccgcgca cgccgcgcac cacccggccg
      661 tgcaccaccc catcctccct cccgcagccg ccgccgccgc cgcagccgcc gccgccgcag
      721 cggtgtccag cgcctctcta ccgggctccg ggctgtcctc ggtcggatcc atccggcctc
      781 cgcacggcct gctcaagtca ccgtcggcag ccgcagccgc ccctctcgga ggcgggggtg
      841 gaggcagcgg tggcagcggt ggcttccagc actggggcgg catgccgtgc ccctgcagca
      901 tgtgccaggt gccgccgccg caccaccacg tgtcggctat gggcgccggc accttgccgc
      961 gcctcacctc cgacgccaag tgagaattcg atatcaagct tatcgataat caacctctgg
     1021 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1081 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1141 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1201 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1261 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     1321 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     1381 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     1441 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     1501 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     1561 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     1621 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     1681 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     1741 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     1801 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     1861 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     1921 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     1981 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     2041 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     2101 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2161 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2221 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2281 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     2341 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2401 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2461 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2521 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     2581 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2641 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2701 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     2761 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2821 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2881 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2941 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3001 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     3061 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     3421 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     3481 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     3541 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     3601 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     3661 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     3721 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3781 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3841 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     3901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3961 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4141 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6301 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     6361 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     6421 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     6481 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     6541 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     6601 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6781 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6841 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6901 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     6961 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     7021 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     7081 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     7141 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7321 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7381 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7441 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7501 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7561 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7621 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7681 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7741 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7801 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7861 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7921 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7981 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8041 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8101 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8161 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8221 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8281 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8341 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8401 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8461 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8521 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8581 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     8641 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     8701 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     8761 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8821 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8881 attacaggga cagcagagat ccagtttggt tactcgagtt taccactccc tatcagtgat
     8941 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9001 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9061 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9121 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9181 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta
     9241 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     9301 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc
     9361 g
//
