LOCUS       sequence-64387-e       10188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-VEcadTS
FEATURES             Location/Qualifiers
     source          1..10188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1566..2165
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1566..1585)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2062..2082
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2340..2643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2644..2847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2798..2818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4926..5633
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(5754..5756,5757..5759,5760..6470)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5799..5820)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6060..6079)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6407..6428
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6927..7520
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7623..7645)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    7773..7806
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7866..7899
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7919..7938
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8019..8036)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8190..8778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8270..8289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8949..9740,9741..9809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9572..9591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9810..9914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9982..10000
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2881 cggactcaga tctcgagctc aagcttgata tcaccatgca gaggctcaca gagctggcca
     2941 ctgctttggg agccttcctg ggcctcctgg cagtggcagc aatggcaggc cctaactttc
     3001 cccagataga cacccccaac atgctacctg cccaccatcg ccaaaagaga gactggattt
     3061 ggaatcaaat gcacatcgat gaagagaaaa atgaatcgct gccccactat gtgggaaaga
     3121 tcaagtccaa cgtgaaccgc cagaatgcta agtatgtgct ccaaggagag tttgctggca
     3181 agatcttcgg ggtcgatgct aacacaggga atgtgcttgc ctatgagagg ctagaccggg
     3241 agaaagtctc tgagtacttc cttactgccc tcattgtgga caagaacacc aacaaaaacc
     3301 tggaacaacc ttccagcttc actgtcaagg tgcatgatat aaatgacaat tggcctgtgt
     3361 tttcgcacca ggtattcaac gcatctgtgc cagagatgtc agctataggg acctctgtca
     3421 tccgtgtgac agcagtggat gcagatgacc ccactgtggc cggccacgcc actgtcttgt
     3481 accaaatcgt gaaaggaaat gagtatttca gcattgataa ttctggactc attttcacaa
     3541 agatcaaaaa cttggaccga gagaaacagg ctgaatacaa gatcgtggtg gaaacacaag
     3601 atgccctggg tctgcgggga gagtcaggca cagccactgt gatgatcaga ctggaggaca
     3661 tcaatgacaa cttccccgtc tttactcaat ccacatacac attttcggtg cctgaagaca
     3721 tccgagtggg caagcccttg ggctttctga ctgttgtgga cccagatgag cctcagaacc
     3781 ggatgaccaa gtacagcatc atgcagggcg agtacaggga caccttcacc attgagacag
     3841 accccaaacg taacgagggt atcatcaaac ccacgaagtc cctggactat gaagtcatcc
     3901 agcagtacac attctacatt gaggccacag accccactat ccgatacgaa tacctgagca
     3961 gcacttcagg caaaaacaaa gccatggtca ccatcaacgt cctagatgtg gatgagcccc
     4021 ctgtcttcca gcgacacttc taccacttca agctgccaga aaaccagaag aaaccgctga
     4081 tcggcactgt ggtggccaaa gaccctgaca aggctcagcg cagcatcggg tactccatcc
     4141 gcaagaccag tgacagaggc caattcttcc gaataaccaa gcagggaaac atctataacg
     4201 agaaagaact ggacagagaa acctacgcct ggtataacct gactgtggag gccaacgaac
     4261 tggattctcg gggtaaccct gtagggaaag agtccattgt gcaggtttac attgaagtct
     4321 tggatgagaa tgacaaccct ccggaatttg cccagcccta cgaacctaaa gtgtgtgaga
     4381 atgctgccca gggcaagctg gtagtacaga tctctgcaac agacaaggat gtggtgccag
     4441 taaacccaaa gttcaagttt gccctgaaga acgaggacag caacttcacc ctcataaaca
     4501 accatgacaa caccgccaac atcacggtca agtatgggca gtttaatcgg gagcatgcca
     4561 agttccacta cctgcccgtg ctcatctcag acaacggggt gcccagcctg actggaacca
     4621 gcacgctaac cgtgggtgtg tgcaagtgca acgagcaggg cgagttcacc ttctgtgagg
     4681 agatggcagc ccaggcgggt gtcagcatcc aggcgctggt ggccatcttc ctctgcatcc
     4741 tcaccatcac agtgattacc ttgctgatca tcctgcggag gcggatccgg aagcaggcgc
     4801 atgctcatag caagagtgcg ctggagattc acgagcagtt ggtcacttac gatgaggagg
     4861 gcggtggtga gatggacacc accagctatg atgtgtcagt gcttaattct gtgcgcggtg
     4921 tcgagatggt gagcaagggc gaggagacca caatgggcgt aatcaagccc gacatgaaga
     4981 tcaagctgaa gatggagggc aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg
     5041 agggcaagcc ctacgacggc accaacacca tcaacctgga ggtgaaggag ggagcccccc
     5101 tgcccttctc ctacgacatt ctgaccaccg cgttcgccta cggcaacagg gccttcacca
     5161 agtaccccga cgacatcccc aactacttca agcagtcctt ccccgagggc tactcttggg
     5221 agcgcaccat gaccttcgag gacaagggca tcgtgaaggt gaagtccgac atctccatgg
     5281 aggaggactc cttcatctac gagatacacc tcaagggcga gaacttcccc cccaacggcc
     5341 ccgtgatgca gaagaagacc accggctggg acgcctccac cgagaggatg tacgtgcgcg
     5401 acggcgtgct gaagggcgac gtcaagcaca agctgctgct ggagggcggc ggccaccacc
     5461 gcgttgactt caagaccatc tacagggcca agaaggcggt gaagctgccc gactatcact
     5521 ttgtggacca ccgcatcgag atcctgaacc acgacaagga ctacaacaag gtgaccgttt
     5581 acgagagcgc cgtggcccgc aactccaccg acggcatgga cgagctgtac aaggggccag
     5641 gtggtgcagg gccaggtggt gcagggccag gtggtgcagg gccaggtggt gcagggcccg
     5701 gtggtgcagg tccaggtggt gcaggtccag gtggtgcagg tccaggtggt gctatggtga
     5761 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5821 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5881 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5941 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     6001 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6061 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6121 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6181 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     6241 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     6301 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6361 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6421 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gcggccgcgg
     6481 gctccactaa gcccctgcgc tccacgatgg acgcccggcc ggcagtgtac acgcaggtgc
     6541 agaagccgcc gcgcctggcg cctggactgc acggagggcc cagggagatg gccaccatga
     6601 tcgacgtgaa gaaagaagag gcagacaatg acggcggtgg ccccccatac gacacactgc
     6661 acatctacgg ctacgagggc gcagagtcca tcgcagagtc cctcagttcc ctgagcacca
     6721 attcctccga ctctgacatc gattatgact tcctcaacga ctggggaccc aggttcaaga
     6781 tgctggctga gctgtacggc tcagatcccc aggaggaact catcatctag gaattcgtta
     6841 ggccattaag gcctgtcgac aagcggccgc ctcggccaaa catcgataaa ataaaagatt
     6901 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     6961 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     7021 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     7081 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     7141 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     7201 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     7261 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     7321 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     7381 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     7441 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     7501 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     7561 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     7621 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7681 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7741 acccagtcac gtagcgatag cggagtgtac tgataacttc gtataatgta tgctatacga
     7801 agttattagg tctgaagagg agtttacgtc cagccaagct agggccgcga tccggaaccc
     7861 ttaatataac ttcgtataat gtatgctata cgaagttatc agtactggct taactatgcg
     7921 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     7981 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9181 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9241 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     9301 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9361 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9421 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9481 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9541 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     9601 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9661 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9721 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9781 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9901 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9961 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattg
    10021 ctagcaattg ctagcaattg ctagcaattc ataccagatc accgaaaact gtcctccaaa
    10081 tgtgtccccc tcacactccc aaattcgcgg gcttctgcct cttagaccac tctaccctat
    10141 tccccacact caccggagcc aaagccgcgg cccttccgtt tctttgct
//
