LOCUS       sequence-64417-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged Elongin B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Mahrour N, Redwine WB, Florens L, Swanson SK, Martin-Brown S, 
            Bradford WD, Staehling-Hampton K, Washburn MP, Conaway RC, Conaway 
            JW
  TITLE     Characterization of Cullin-box sequences that direct recruitment of 
            Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin 
            ligases.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):8005-13. Epub 2008 Jan 10.
  PUBMED    18187417
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-03-21"; volume: "283(12)"; pages: "8005-13. Epub
            2008 Jan 10"
COMMENT     Sequence Label: pcDNA5/FRT-Flag-EloB
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..26
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(449..466)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    455..679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(812..831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atggattaca ggatgacgac gataagctcg agatggacgt gtttctcatg atccggcgcc
       61 acaagaccac catctttacg gacgccaagg agtcgagcac ggtgttcgaa ctgaagcgca
      121 tcgtcgaggg catcctcaag cggccgccag aggagcagcg gctgtacaag gacgaccagc
      181 tccttgatga tggaaaaact ctgggcgaat gtggcttcac cagtcagaca gcaaggccac
      241 aggccccagc cacagtgggc ctggcctttc gagcagatga cacctttgaa gcgctgcgta
      301 ttgaaccctt ctccagccct ccggagcttc cagatgtgat gaagccacag gattctggag
      361 gcagtgccaa tgaacaagct gtgcagtgag aattccctgc agcgcggccg cgtcgagtct
      421 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      481 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      541 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      601 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      661 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
      721 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc
      961 tagaagttcc tattccgaag t
//
