LOCUS       sequence-64418-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Chong T, Collins JJ 3rd, Brubacher JL, Zarkower D, Newmark PA
  TITLE     A sex-specific transcription factor controls male identity in a 
            simultaneous hermaphrodite.
  JOURNAL   Nat Commun. 2013; 4:1814. doi: 10.1038/ncomms2811.
  PUBMED    23652002
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: " 4:1814. doi"; pages: " 
            10.1038/ncomms2811"
COMMENT     Sequence Label: pTC7-dmd-1
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(211..229)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(212..229)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      265..312
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(324..341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(575..594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaattaata cgactcacta taggtttttt tttttttgtg gcctttccaa gcattgtgtt
       61 ctccatgatt tctgcatcgt ctacaatgtg gtgattgcgg agaccttttg ctgatgtctt
      121 tctgatttct ataaagtcta atttgacgag cgacaatttc tcgaccatga ctaacgactg
      181 tacacaatgg acagggacac aaaggtcact tctatagtgt cacctaaatc tccctatagt
      241 gaggggtatt tggatgcggc gcgcctggca taaccccttg gggcctctaa acgggtcttg
      301 aggggttttt tgacgcgtgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gta
//
