LOCUS       sequence-64438-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Oliveira AF, Yasuda R
  TITLE     An improved Ras sensor for highly sensitive and quantitative 
            FRET-FLIM imaging.
  JOURNAL   PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 
            2013 Jan 14.
  PUBMED    23349692
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e52874. doi"; pages: " 
            10.1371/journal.pone.0052874. Epub 2013 Jan 14"
COMMENT     Sequence Label: FRas2-M Acceptor
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..684)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(7..684)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(69..92)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(267..286)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     536..554
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggccgcttag gcgccggtgg agtggcggcc ctcggcgcgc tcgtactgtt ccacgatggt
       61 gtagtcctcg ttgtgggagg tgatgtccag cttgatgtcg gtcttgtagg cgccgggcag
      121 ctgcacgggc ttcttggcca tgtaggtggt cttgacctcg gcgtcgtagt ggccgccgtc
      181 cttcagcttc agcctcatct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
      241 ctcggtggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      301 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
      361 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      421 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
      481 gccgtactgg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt
      541 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
      601 ctcgaactcg tggccgttca cggagccctc catgcgcacc ttgaagcgca tgaactcctt
      661 gatgacgtcc tcggaggagg ccatt
//
