LOCUS       sequence-64444-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Sathiamoorthy S, Shin JA
  TITLE     Boundaries of the origin of replication: creation of a 
            pET-28a-derived vector with p15A copy control allowing compatible 
            coexistence with pET vectors.
  JOURNAL   PLoS One. 2012;7(10):e47259. doi: 10.1371/journal.pone.0047259. Epub
            2012 Oct 22.
  PUBMED    23110063
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47259. doi"; pages: " 
            10.1371/journal.pone.0047259. Epub 2012 Oct 22"
COMMENT     Sequence Label: pSAM
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(389..406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(603..622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
       61 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
      121 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tcctggcttc
      181 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
      241 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
      301 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
      361 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      421 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
      481 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
      541 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
      601 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
      661 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
      721 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
      841 aagcaccact ggcagcagcc actggtaatt gattttagag gagttagtct tgaagtcatg
      901 cgccggttaa ggcta
//
