LOCUS       sequence-64446-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP1
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..128)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    138..156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    159..177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(179..382)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(208..228)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        383..762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcccttgct caccatctta agtttaaacg ctagagtccg gaggctggat cggtcccggt
       61 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
      121 aacgagctcg tcgacgatct ctatcactga tagggagatc tctatcactg atagggagag
      181 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      241 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      301 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      361 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      421 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      481 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      541 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      601 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      661 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      721 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
      781 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
      841 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
      901 cacaa
//
