LOCUS       sequence-64448-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP1
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(304..348)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(361..381)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(436..457)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     785..804
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt ttgatgaccg ttatagttaa tacctccacc
      301 tccgctgtcc atgtgctggc gttcgaattt agcagcagcg gtttctttcc cgcccccgcc
      361 gccctgaaaa taaagattct ccccgccccc gcccttgtac agctcgtcca tgccgagagt
      421 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      481 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      541 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      601 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      661 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      721 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      781 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      841 gggcatggcg gacttgaaga agtcgtgctg cttcatgt
//
