LOCUS       sequence-64456-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged Cul5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Mahrour N, Redwine WB, Florens L, Swanson SK, Martin-Brown S, 
            Bradford WD, Staehling-Hampton K, Washburn MP, Conaway RC, Conaway 
            JW
  TITLE     Characterization of Cullin-box sequences that direct recruitment of 
            Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin 
            ligases.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):8005-13. Epub 2008 Jan 10.
  PUBMED    18187417
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-03-21"; volume: "283(12)"; pages: "8005-13. Epub
            2008 Jan 10"
COMMENT     Sequence Label: pcDNA5/FRT-Flag-Cul5
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggattacaag gatgacgacg ataagctcga gatggcgacg tctaatctgt taaagaataa
       61 aggttctctt cagtttgaag acaaatggga ttttatgcgc ccgattgttt tgaagctttt
      121 acgccaggaa tctgttacaa aacagcagtg gtttgatctg ttttcggatg tgcatgcagt
      181 ctgtctttgg gatgataaag gcccagcaaa aattcatcag gctttaaaag aagatattct
      241 tgagtttatt aagcaggcac aggcacgagt actgagccat caagatgata cggctttgct
      301 aaaagcatat attgttgaat ggcgaaagtt ctttacacaa tgtgatattt taccaaaacc
      361 tttttgtcaa ctagagatta ctttaatggg taaacagggc agcaataaaa aatcaaatgt
      421 ggaagacagt attgttcgaa agcttatgct tgatacatgg aatgagtcaa tcttttcaaa
      481 cataaaaaac agactccaag atagtgcaat gaagctggta catgctgaga gattgggaga
      541 agcttttgat tctcagctgg ttattggagt aagagaatcc tatgttaacc tttgttctaa
      601 tcctgaggat aaacttcaaa tttataggga caattttgag aaggcatact tggattcaac
      661 agagagattt tatagaacac aagcaccctc gtatttacaa caaaatggtg tacagaatta
      721 tatgaaatat gcagatgcta aattaaaaga agaagaaaaa cgagcactac gttatttaga
      781 aacaagacga gaatgtaact ccgttgaagc actcatggaa tgctgtgtaa atgccctggt
      841 gacatcattt aaagagacta tcttagctga gtgccaaggc atgatcaaga gaaatgaaac
      901 tgaaaaatta catttaatgt tttcattgat ggacaaagtt cctaatggta tagagccaat
      961 gttgaaagac ttggagga
//
