LOCUS       sequence-64471-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP4
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(118..242)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             295..336
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(374..391)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    380..604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggggagagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
       61 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
      121 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      181 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
      241 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
      301 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttaa
      361 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
      601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcgggggctc ccttta
//
