LOCUS       sequence-64473-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP4
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(307..351)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(364..384)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(400..423)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(458..477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          503..706
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(526..546)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt tgatatctgc agaattccac cacactggac
      301 tagtccgctg tccatgtgct ggcgttcgaa tttagcagca gcggtttctt tcccgccccc
      361 gccgccctga aaataaagat tctccccgcc cccgcctgcc ttatcgtcgt catccttgta
      421 atccatggta ccaagcttac ctagccagct tgggtctccc tatagtgagt cgtattagta
      481 ccaagctaat tcctcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
      541 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
      601 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
      661 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
      721 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctg
//
