LOCUS       sequence-64476-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Rouhana L, Weiss JA, King RS, Newmark PA
  TITLE     PIWI homologs mediate histone H4 mRNA localization to planarian 
            chromatoid bodies.
  JOURNAL   Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. 
            Epub 2014 Jun 5.
  PUBMED    24903754
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.101618"; journalName: "Development"; date: "2014-07"; 
            volume: "141"; issue: "13"; pages: "2592-601"
COMMENT     Sequence Label: pBS-Histone H3
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     33..49
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(644..664)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(644..662)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(696..718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    707..723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(731..737,738..755,756..761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    776..797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat
       61 atcgaattcc attgatgttg ggggctcgaa ctaaacagac tgcgagaaaa agtactggtg
      121 gaaaagctcc aagaaagcag ttggcaacta aagctgcacg taaaagtgca cctgcaactg
      181 gtggtgttaa gaaaccacat agatatcgtc caggaactgt agctctaaga gaaattcgta
      241 ggtatcaaaa gagtactgaa ttgttgatca gaaagttgcc ctttcagcgt cttgtgcgag
      301 aaatcgctca agatttcaag acggatttgc gatttcaaag ttcagctgtt tctgctcttc
      361 aagaggctag tgaagcatac ttagttggtt tgtttgaaga tactaatttg tgtgcaattc
      421 acgctaaacg tgttaccatt atgccgaagg atatccattt agctagaaga ataagaggag
      481 aacgtgcata aggtgttatg tgagaatgcg ttacggccct ctttagggcc acaagctttg
      541 gttgtaaata gctattattg attaatagag tgttatatat tcacaaaaaa aaaaaaaaaa
      601 aatgcgagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
      661 ttgcgcgctt ggcgtaatca tggtcatacc tgtttcctgt gtgaaattgt tatccgctca
      721 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      781 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      841 c
//
