LOCUS       sequence-64497-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Liu D, Davydenko O, Lampson MA
  TITLE     Polo-like kinase-1 regulates kinetochore-microtubule dynamics and 
            spindle checkpoint silencing.
  JOURNAL   J Cell Biol. 2012 Aug 20;198(4):491-9. doi: 10.1083/jcb.201205090.
  PUBMED    22908307
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-08-20- 20"; volume: "198"; 
            issue: "4"; pages: "491-9"
COMMENT     Sequence Label: Plk1 FRET sensor c-jun substrate
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(191..703,704..706))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     384..403
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     643..664
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctcgttcat gccttcagtg atcccggcgg cggtcaggaa ctccagcagg accatgtgat
       61 cgcgcttctc gttggggtct ttgaacaggg cggactggta gctcaggtag tggttgtcgg
      121 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      181 cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
      241 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      301 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      361 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      421 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      481 cggggtagcg ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg
      541 gcacgggcag cttgccggtg gtgcagagca gcttcagggt cagcttgccg taggtggcat
      601 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      661 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcactcct ccggagccct
      721 cggagggcag gaaggtggcg ttcagggcgt cgtcggatcc tcctaacttt ttcaccaaat
      781 ctttttcttc ggctgtttgc ttaagtacta ctctttgttc ttgtaacata cctaatccct
      841 cgttaaacag acctgtagta tcgttaattt ccactttaaa gccaatgaca aatttattgt
      901 ttttatccca a
//
