LOCUS       sequence-64506-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann
            P, Brautigan DL, Stukenberg PT, Kapoor TM
  TITLE     Midzone activation of aurora B in anaphase produces an intracellular
            phosphorylation gradient.
  JOURNAL   Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub
            2008 May 7.
  PUBMED    18463638
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature06923"; journalName: "Nature"; date: "2008-06-19- 
            19"; volume: "453"; issue: "7198"; pages: "1132-6"
COMMENT     Sequence Label: CENP-B targeted Aurora B FRET sensor Kif2 subs...
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      613..622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggcgc gccgccacca tgggccccaa gaggcgacag
      121 ctgacgttcc gggagaagtc acggatcatc caggaggtgg aggagaatcc ggacctgcgc
      181 aagggcgaga tcgcgcggcg cttcaacatc ccgccgtcca cgctgagcac gatcctgaag
      241 aacaagcgcg ccatcctggc gtcggagcgc aagtacgggg tggcctccac ctgccgcaag
      301 accaacaagc tgtctcccta cgacaagctc gagggcttgc tcatcgcctg gttccagcag
      361 atccgcgccg ccggcctgcc ggtcaagggc atcatcctca aggagaaggc gctgcgcata
      421 gccgaggagc tgggcatgga cgacttcacc gcctccaacg gctggctgga ccgcttccgc
      481 cggcgccacg gcgtggtgtc ctgcagcggc gtggcccgcg cccgcgcgcg aaacgctgcc
      541 ccccgcaccc cggcggcgcc tgccagtccg gccgcggtgc cctcggaggg cagtggcggg
      601 ctagcaagct tcgccaccat ggtgagcaag ggcgaggagc tgttcggcgg gatcgtgccc
      661 atcctggtcg agctggaggg cgacgtaaac ggccacaggt tcagcgtgtc cggcgagggc
      721 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      781 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
      841 taccccgacc acatgaagca gcacgacttc ttcaagtccg tcatgcccga aggctacgtc
      901 caggagcgta ccatcttctt caaggacgac ggcaactac
//
